Restriction map for
pF1KB7624
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 48 CCGC CCGC(-3/-1)
AfaI 70 GTAC GT^AC
AspHI 91 GAGCTC G[AT]GC[AT]^C
AvaII 486 GGACC G^G[AT]CC
BanII 91 GAGCTC G[GA]GC[CT]^C
BbvCI 612 CCTCAGC CCTCAGC(-5/-2)
BclI 552 TGATCA T^GATCA
BcnI 13 CCCGG CC^[GC]GG
BlpI 42 GCTCAGC GC^T[ACGT]AGC
BmyI 91 GAGCTC G[AGT]GC[ACT]^C
BpmI 96 CTGGAG CTGGAG(16/14)
Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC
BsaBI 553 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 982 GTGCAG GTGCAG(16/14)
BsiHKAI 91 GAGCTC G[AT]GC[AT]^C
BsmBI 58 GAGACG CGTCTC(1/5)
Bsp1286I 91 GAGCTC G[AGT]GC[ACT]^C
BspHI 549 TCATGA T^CATGA
BstNI 54 CCAGG CC^[AT]GG
Cac8I 17 GCCTGC GC[ACGT]^[ACGT]GC
CelII 42 GCTCAGC GC^T[ACGT]AGC
CfoI 37 GCGC GCG^C
Cfr13I 486 GGACC G^G[ACGT]CC
DraI 321 TTTAAA TTT^AAA
DraII 485 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 485 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 54 CCAGG ^CC[AT]GG
FbaI 552 TGATCA T^GATCA
HaeII 36 GGCGCT [GA]GCGC^[CT]
HapII 14 CCGG C^CGG
HhaI 37 GCGC GCG^C
HinP1I 37 GCGC G^CGC
HincII 272 GTCAAC GT[CT]^[GA]AC
HindII 272 GTCAAC GT[CT]^[GA]AC
HindIII 170 AAGCTT A^AGCTT
HpaII 14 CCGG C^CGG
HphI 456 TCACC GGTGA(8/7)
MaeIII 469 GTTAC ^GT[ACGT]AC
MamI 553 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 355 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 14 CCGG C^CGG
MvaI 54 CCAGG CC^[AT]GG
MwoI 420 GCCCAAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 13 CCCGG CC^[GC]GG
NlaIV 486 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 485 AGGACCC [GA]G^G[AT]CC[CT]
PstI 19 CTGCAG CTGCA^G
RcaI 549 TCATGA T^CATGA
RsaI 70 GTAC GT^AC
SacI 91 GAGCTC GAGCT^C
Sau96I 486 GGACC G^G[ACGT]CC
SfcI 19 CTGCAG C^T[GA][CT]AG
SspI 643 AATATT AAT^ATT
StuI 803 AGGCCT AGG^CCT
XcmI 216 CCAGATCAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 155 ACATGT A^C[GA][CT]GT
573 ACATGT
BbvI 364 GCAGC GCAGC(8/12)
39 GCTGC
BfaI 391 CTAG C^TAG
911 CTAG
BseRI 8 CTCCTC GAGGAG(10/8)
1092 GAGGAG
BsmAI 58 GAGAC GTCTC(1/5)
960 GTCTC
BsmFI 4 GGGAC GGGAC(10/14)
115 GGGAC
BspLU11I 155 ACATGT A^CATGT
573 ACATGT
BsrDI 415 GCAATG GCAATG(2/0)
977 GCAATG
BssKI 13 CCCGG ^CC[ACGT]GG
54 CCAGG
HaeIII 16 GGCC GG^CC
804 GGCC
MaeI 391 CTAG C^TAG
911 CTAG
NspI 155 ACATGT [GA]CATG^[CT]
573 ACATGT
ScrFI 13 CCCGG CC^[ACGT]GG
54 CCAGG
TaqI 77 TCGA T^CGA
860 TCGA
TseI 39 GCTGC G^C[AT]GC
364 GCAGC
TthHB8I 77 TCGA T^CGA
860 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI BsiYI BslI
BstF5I DdeI DpnII EarI Fnu4HI
FokI HinfI ItaI Ksp632I MboI
MboII MnlI MseI NdeII NlaIII
PleI Sau3AI SfaNI TfiI Tru9I
Tsp509I TspRI