Restriction map for
pF1KB7623
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DsaI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MflI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfaNI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 817 GTCTAC GT^[AC][GT]AC
AlwNI 451 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbrPI 828 CACGTG CAC^GTG
BpmI 28 CTGGAG CTGGAG(16/14)
BsgI 502 GTGCAG GTGCAG(16/14)
BsiEI 52 CGGCCG CG[GA][CT]^CG
BsmFI 824 GGGAC GGGAC(10/14)
BstAPI 221 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 557 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 642 CTCAG C^T[ACGT]AG
DpnII 363 GATC ^GATC
DrdI 669 GACCATGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoNI 462 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 191 CCAAGG C^C[AT][AT]GG
HinfI 145 GAGTC G^A[ACGT]TC
MaeIII 446 GTAAC ^GT[ACGT]AC
MboI 363 GATC ^GATC
MluI 923 ACGCGT A^CGCGT
NaeI 529 GCCGGC GCC^GGC
NdeII 363 GATC ^GATC
NgoMIV 529 GCCGGC G^CCGGC
NspI 707 GCATGC [GA]CATG^[CT]
PleI 145 GAGTC GAGTC(4/5)
PmaCI 828 CACGTG CAC^GTG
PmlI 828 CACGTG CAC^GTG
PstI 898 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
Sau3AI 363 GATC ^GATC
SbfI 897 CCTGCAGG CCTGCA^GG
SfiI 895 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 707 GCATGC GCATG^C
Sse8387I 897 CCTGCAGG CCTGCA^GG
StyI 191 CCAAGG C^C[AT][AT]GG
TspRI 207 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 374 GGACC G^G[AT]CC
459 GGACC
BanI 567 GGCGCC G^G[CT][GA]CC
694 GGCGCC
BbeI 567 GGCGCC GGCGC^C
694 GGCGCC
BsaAI 478 TACGTG [CT]AC^GT[GA]
828 CACGTG
BsiWI 277 CGTACG C^GTACG
788 CGTACG
BsrFI 529 GCCGGC [GA]^CCGG[CT]
745 ACCGGC
BsrI 548 ACTGG ACTGG(1/-1)
930 CCAGT
Cfr10I 529 GCCGGC [GA]^CCGG[CT]
745 ACCGGC
EaeI 52 CGGCCG [CT]^GGCC[GA]
764 CGGCCA
HgaI 4 GACGC GACGC(5/10)
815 GCGTC
KasI 567 GGCGCC G^GCGCC
694 GGCGCC
MslI 345 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
666 CATGACCATG
NarI 567 GGCGCC GG^CGCC
694 GGCGCC
PaeR7I 127 CTCGAG C^TCGAG
837 CTCGAG
PflMI 72 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGCAGCTGG
SfcI 810 CTACAG C^T[GA][CT]AG
898 CTGCAG
SfoI 567 GGCGCC GGC^GCC
694 GGCGCC
TaqI 128 TCGA T^CGA
838 TCGA
TthHB8I 128 TCGA T^CGA
838 TCGA
Van91I 72 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGCAGCTGG
XhoI 127 CTCGAG C^TCGAG
837 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI ApaI AvaI BanII BbvI
BcnI BfaI BglI BmyI BsaHI
BsaJI BseRI BsiYI BslI BsoBI
Bsp1286I BsrBI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SmlI TseI