##################################
# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AseI 43 ATTAAT AT^TAAT
AsnI 43 ATTAAT AT^TAAT
BanII 214 GAGCTC G[GA]GC[CT]^C
BclI 582 TGATCA T^GATCA
BfaI 553 CTAG C^TAG
BglII 858 AGATCT A^GATCT
BpmI 706 CTGGAG CTGGAG(16/14)
BseRI 709 GAGGAG GAGGAG(10/8)
BsgI 760 CTGCAC GTGCAG(16/14)
BsiYI 447 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 447 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstF5I 537 GGATG GGATG(2/0)
BstYI 858 AGATCT [GA]^GATC[CT]
Cac8I 125 GCAGGC GC[ACGT]^[ACGT]GC
EarI 475 GAAGAG CTCTTC(1/4)
EcoNI 447 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 582 TGATCA T^GATCA
FokI 537 GGATG GGATG(9/13)
HphI 146 TCACC GGTGA(8/7)
Ksp632I 475 GAAGAG CTCTTC(1/4)
MaeI 553 CTAG C^TAG
MflI 858 AGATCT [GA]^GATC[CT]
MslI 28 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 523 GCTACAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 83 GACTC GAGTC(4/5)
PshBI 43 ATTAAT AT^TAAT
SacI 214 GAGCTC GAGCT^C
TaqI 813 TCGA T^CGA
TthHB8I 813 TCGA T^CGA
XhoII 858 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 286 CTTAAG C^TTAAG
621 CTTAAG
AspHI 22 GAGCAC G[AT]GC[AT]^C
214 GAGCTC
BbsI 504 GAAGAC GAAGAC(2/6)
669 GAAGAC
BfrI 286 CTTAAG C^TTAAG
621 CTTAAG
BmyI 22 GAGCAC G[AGT]GC[ACT]^C
214 GAGCTC
BpuAI 504 GAAGAC GAAGAC(2/6)
669 GAAGAC
BsiHKAI 22 GAGCAC G[AT]GC[AT]^C
214 GAGCTC
BsmAI 81 GAGAC GTCTC(1/5)
160 GTCTC
Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C
214 GAGCTC
Cfr13I 12 GGGCC G^G[ACGT]CC
612 GGCCC
HaeIII 13 GGCC GG^CC
612 GGCC
MspA1I 256 CAGCTG C[AC]G^C[GT]G
757 CAGCTG
NlaIII 765 CATG CATG^
819 CATG
PvuII 256 CAGCTG CAG^CTG
757 CAGCTG
Sau96I 12 GGGCC G^G[ACGT]CC
612 GGCCC
SfcI 92 CTGTAG C^T[GA][CT]AG
292 CTACAG
Tsp45I 397 GTCAC ^GT[GC]AC
702 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsrI
DdeI DpnII Fnu4HI HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII Sau3AI SmlI TfiI Tru9I
TseI Tsp509I TspRI