Restriction map for
pF1KB7589
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SalI
SapI ScaI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AflIII 323 ACACGT A^C[GA][CT]GT
Alw44I 319 GTGCAC G^TGCAC
AocI 565 CCTGAGG CC^T[ACGT]AGG
Aor51HI 281 AGCGCT AGC^GCT
ApaI 598 GGGCCC GGGCC^C
ApaLI 319 GTGCAC G^TGCAC
BamHI 502 GGATCC G^GATCC
BbrPI 336 CACGTG CAC^GTG
BbvCI 592 GCTGAGG CCTCAGC(-5/-2)
BfaI 458 CTAG C^TAG
BglI 562 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 336 CACGTG [CT]AC^GT[GA]
BseRI 556 GAGGAG GAGGAG(10/8)
BsgI 169 GTGCAG GTGCAG(16/14)
BsmAI 61 GAGAC GTCTC(1/5)
BsmBI 61 GAGACG CGTCTC(1/5)
BsrFI 292 GCCGGC [GA]^CCGG[CT]
BsrI 303 CCAGT ACTGG(1/-1)
BssSI 313 CACGAG CACGAG(-5/-1)
Bsu36I 565 CCTGAGG CC^T[ACGT]AGG
Cfr10I 292 GCCGGC [GA]^CCGG[CT]
DraIII 313 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 352 GACGCTACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 11 CCGCGG C^C[GA][CT]GG
EaeI 21 TGGCCG [CT]^GGCC[GA]
Eco47III 281 AGCGCT AGC^GCT
Eco81I 565 CCTGAGG CC^T[ACGT]AGG
EcoNI 552 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 667 CCTTGG C^C[AT][AT]GG
KspI 11 CCGCGG CCGC^GG
MaeI 458 CTAG C^TAG
MboII 102 GAAGA GAAGA(8/7)
MslI 324 CACGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 292 GCCGGC GCC^GGC
NgoMIV 292 GCCGGC G^CCGGC
NspI 308 GCATGC [GA]CATG^[CT]
PaeR7I 388 CTCGAG C^TCGAG
PmaCI 336 CACGTG CAC^GTG
PmlI 336 CACGTG CAC^GTG
PpuMI 525 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 259 CAGCTG CAG^CTG
SacI 370 GAGCTC GAGCT^C
SacII 11 CCGCGG CCGC^GG
SbfI 132 CCTGCAGG CCTGCA^GG
SmaI 522 CCCGGG CCC^GGG
SmlI 388 CTCGAG C^T[CT][GA]AG
SphI 308 GCATGC GCATG^C
Sse8387I 132 CCTGCAGG CCTGCA^GG
StuI 664 AGGCCT AGG^CCT
StyI 667 CCTTGG C^C[AT][AT]GG
Tsp509I 645 AATT ^AATT
TspRI 304 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 388 CTCGAG C^TCGAG
XmaCI 522 CCCGGG C^CCGGG
XmaI 522 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 136 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
424 CAGGATCTG
AvaI 388 CTCGAG C^[CT]CG[GA]G
522 CCCGGG
BsoBI 388 CTCGAG C^[CT]CG[GA]G
522 CCCGGG
BstAPI 135 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
312 GCACGAGGTGC
BstF5I 300 CATCC GGATG(2/0)
45 GGATG
BstYI 426 GGATCT [GA]^GATC[CT]
502 GGATCC
FokI 300 CATCC GGATG(9/13)
45 GGATG
HinfI 391 GAGTC G^A[ACGT]TC
579 GAGTC
MaeII 325 ACGT A^CGT
337 ACGT
MflI 426 GGATCT [GA]^GATC[CT]
502 GGATCC
NlaIII 309 CATG CATG^
396 CATG
PleI 391 GAGTC GAGTC(4/5)
579 GAGTC
PstI 133 CTGCAG CTGCA^G
262 CTGCAG
SfcI 133 CTGCAG C^T[GA][CT]AG
262 CTGCAG
TaqI 350 TCGA T^CGA
389 TCGA
TthHB8I 350 TCGA T^CGA
389 TCGA
XhoII 426 GGATCT [GA]^GATC[CT]
502 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI SfoI
TseI