Restriction map for
pF1KB7588
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 66 GACGTC GACGT^C
AspHI 610 GAGCAC G[AT]GC[AT]^C
AvaII 24 GGTCC G^G[AT]CC
BanI 316 GGCGCC G^G[CT][GA]CC
BbeI 316 GGCGCC GGCGC^C
BfaI 293 CTAG C^TAG
BglI 502 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 696 GCTCAGC GC^T[ACGT]AGC
BsgI 785 GTGCAG GTGCAG(16/14)
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
BsmBI 89 CGTCTC CGTCTC(1/5)
BspHI 584 TCATGA T^CATGA
BspMI 809 ACCTGC ACCTGC(4/8)
BsrBI 690 CCGCTC CCGCTC(-3/-3)
BstYI 565 AGATCC [GA]^GATC[CT]
CelII 696 GCTCAGC GC^T[ACGT]AGC
Cfr13I 24 GGTCC G^G[ACGT]CC
DraII 23 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 232 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 16 GACAGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 23 GGGTCCC [GA]G^G[ACGT]CC[CT]
HindIII 269 AAGCTT A^AGCTT
KasI 316 GGCGCC G^GCGCC
MaeI 293 CTAG C^TAG
MaeII 67 ACGT A^CGT
MflI 565 AGATCC [GA]^GATC[CT]
MslI 706 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 316 GGCGCC GG^CGCC
NheI 292 GCTAGC G^CTAGC
PpuMI 23 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 442 CAGCTG CAG^CTG
RcaI 584 TCATGA T^CATGA
Sau96I 24 GGTCC G^G[ACGT]CC
SfoI 316 GGCGCC GGC^GCC
Tsp45I 364 GTGAC ^GT[GC]AC
Tsp509I 802 AATT ^AATT
XhoII 565 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 328 CGCG CG^CG
499 CGCG
AcyI 66 GACGTC G[GA]^CG[CT]C
316 GGCGCC
AlwI 38 GATCC GGATC(4/5)
566 GATCC
AvaI 112 CCCGAG C^[CT]CG[GA]G
373 CCCGAG
BbsI 471 GAAGAC GAAGAC(2/6)
117 GTCTTC
BbvI 73 GCAGC GCAGC(8/12)
787 GCAGC
BpuAI 471 GAAGAC GAAGAC(2/6)
117 GTCTTC
BsaHI 66 GACGTC G[GA]^CG[CT]C
316 GGCGCC
BsmAI 90 GTCTC GTCTC(1/5)
657 GTCTC
BsoBI 112 CCCGAG C^[CT]CG[GA]G
373 CCCGAG
BstUI 328 CGCG CG^CG
499 CGCG
BstXI 661 CCAGCGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
749 CCAGCTACCTGG
EaeI 446 TGGCCG [CT]^GGCC[GA]
509 TGGCCG
HgaI 329 GCGTC GACGC(5/10)
745 GCGTC
Hin1I 66 GACGTC G[GA]^CG[CT]C
316 GGCGCC
MaeIII 364 GTGAC ^GT[ACGT]AC
644 GTAAC
MvnI 328 CGCG CG^CG
499 CGCG
NlaIII 585 CATG CATG^
712 CATG
PleI 474 GACTC GAGTC(4/5)
115 GAGTC
SfcI 141 CTACAG C^T[GA][CT]AG
282 CTATAG
TfiI 97 GAATC G^A[AT]TC
130 GATTC
TseI 73 GCAGC G^C[AT]GC
787 GCAGC
XcmI 434 CCAGCTTTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
626 CCAGCGACCCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BanII BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV RsaI Sau3AI
ScrFI SfaNI