Restriction map for
pF1KB7583
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsgI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 269 GTCGAC GT^[AC][GT]AC
AccIII 422 TCCGGA T^CCGGA
AlwNI 28 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 458 AGCGCT AGC^GCT
AspHI 501 GAGCAC G[AT]GC[AT]^C
AvaII 256 GGACC G^G[AT]CC
BamHI 419 GGATCC G^GATCC
BanI 246 GGTGCC G^G[CT][GA]CC
BanII 206 GGGCTC G[GA]GC[CT]^C
BsaBI 18 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 658 GGTCTC GGTCTC(1/5)
BsaWI 422 TCCGGA [AT]^CCGG[AT]
BseAI 422 TCCGGA T^CCGGA
BseRI 450 CTCCTC GAGGAG(10/8)
BsiEI 44 CGGCCG CG[GA][CT]^CG
BsiHKAI 501 GAGCAC G[AT]GC[AT]^C
BsiYI 128 CCTTAAAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 128 CCTTAAAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 447 CGTCTC CGTCTC(1/5)
BsmFI 255 GGGAC GGGAC(10/14)
BspEI 422 TCCGGA T^CCGGA
BssKI 225 CCTGG ^CC[ACGT]GG
BstNI 225 CCTGG CC^[AT]GG
BstYI 419 GGATCC [GA]^GATC[CT]
Cac8I 572 GCATGC GC[ACGT]^[ACGT]GC
CfoI 459 GCGC GCG^C
Cfr13I 256 GGACC G^G[ACGT]CC
DraII 255 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 44 CGGCCG [CT]^GGCC[GA]
EagI 44 CGGCCG C^GGCCG
EarI 387 GAAGAG CTCTTC(1/4)
EclXI 44 CGGCCG C^GGCCG
Eco47III 458 AGCGCT AGC^GCT
Eco52I 44 CGGCCG C^GGCCG
EcoO109I 255 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 225 CCTGG ^CC[AT]GG
HaeII 458 AGCGCT [GA]GCGC^[CT]
HaeIII 45 GGCC GG^CC
HapII 423 CCGG C^CGG
HhaI 459 GCGC GCG^C
HinP1I 459 GCGC G^CGC
HpaII 423 CCGG C^CGG
Ksp632I 387 GAAGAG CTCTTC(1/4)
MamI 18 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 419 GGATCC [GA]^GATC[CT]
MroI 422 TCCGGA T^CCGGA
MspA1I 394 CAGCTG C[AC]G^C[GT]G
MspI 423 CCGG C^CGG
MvaI 225 CCTGG CC^[AT]GG
NspI 572 GCATGC [GA]CATG^[CT]
PpuMI 255 GGGACCC [GA]G^G[AT]CC[CT]
PstI 631 CTGCAG CTGCA^G
PvuII 394 CAGCTG CAG^CTG
SalI 269 GTCGAC G^TCGAC
Sau96I 256 GGACC G^G[ACGT]CC
ScrFI 225 CCTGG CC^[ACGT]GG
SfcI 631 CTGCAG C^T[GA][CT]AG
SphI 572 GCATGC GCATG^C
TfiI 262 GAATC G^A[AT]TC
Tsp509I 305 AATT ^AATT
XhoII 419 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 420 GATCC GGATC(4/5)
419 GGATC
BmyI 206 GGGCTC G[AGT]GC[ACT]^C
501 GAGCAC
BsaJI 452 CCTCGG C^C[ACGT][ACGT]GG
582 CCTCGG
BsmAI 448 GTCTC GTCTC(1/5)
659 GTCTC
Bsp1286I 206 GGGCTC G[AGT]GC[ACT]^C
501 GAGCAC
BsrBI 58 GAGCGG CCGCTC(-3/-3)
562 GAGCGG
BsrI 252 ACTGG ACTGG(1/-1)
530 ACTGG
FauI 63 GCGGG CCCGC(4/6)
564 GCGGG
HincII 269 GTCGAC GT[CT]^[GA]AC
490 GTTGAC
HindII 269 GTCGAC GT[CT]^[GA]AC
490 GTTGAC
HphI 520 GGTGA GGTGA(8/7)
348 TCACC
MseI 130 TTAA T^TAA
292 TTAA
MslI 24 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
82 CATGGTTATG
PleI 466 GACTC GAGTC(4/5)
553 GACTC
Tru9I 130 TTAA T^TAA
292 TTAA
Tsp45I 332 GTGAC ^GT[GC]AC
641 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BpmI BstF5I
DpnII Fnu4HI FokI HinfI ItaI
MaeII MaeIII MboI MboII MnlI
MwoI NdeII NlaIII NlaIV Sau3AI
SfaNI TaqI TseI TspRI TthHB8I