Restriction map for
pF1KB7574
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BspEI BspHI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CfoI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tth111I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AcsI 220 AAATTT [GA]^AATT[CT]
AfaI 142 GTAC GT^AC
Alw44I 664 GTGCAC G^TGCAC
AlwNI 496 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 790 CCTGAGG CC^T[ACGT]AGG
ApaLI 664 GTGCAC G^TGCAC
ApoI 220 AAATTT [GA]^AATT[CT]
AseI 124 ATTAAT AT^TAAT
AsnI 124 ATTAAT AT^TAAT
AvaI 247 CCCGGG C^[CT]CG[GA]G
BanI 74 GGTGCC G^G[CT][GA]CC
BbsI 649 GAAGAC GAAGAC(2/6)
BbvCI 808 CCTCAGC CCTCAGC(-5/-2)
BfaI 538 CTAG C^TAG
BglII 238 AGATCT A^GATCT
BlpI 493 GCTCAGC GC^T[ACGT]AGC
BpmI 611 CTCCAG CTGGAG(16/14)
Bpu1102I 493 GCTCAGC GC^T[ACGT]AGC
BpuAI 649 GAAGAC GAAGAC(2/6)
BsiEI 13 CGGCCG CG[GA][CT]^CG
BsiYI 48 CCCGACGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 48 CCCGACGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 58 GAGACG CGTCTC(1/5)
BsmFI 754 GGGAC GGGAC(10/14)
BsmI 569 GCATTC GAATGC(1/-1)
BsoBI 247 CCCGGG C^[CT]CG[GA]G
Bsp1407I 141 TGTACA T^GTACA
BspDI 691 ATCGAT AT^CGAT
BsrFI 15 GCCGGC [GA]^CCGG[CT]
BsrGI 141 TGTACA T^GTACA
BstNI 42 CCTGG CC^[AT]GG
BstXI 86 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 790 CCTGAGG CC^T[ACGT]AGG
CelII 493 GCTCAGC GC^T[ACGT]AGC
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
ClaI 691 ATCGAT AT^CGAT
EaeI 13 CGGCCG [CT]^GGCC[GA]
EagI 13 CGGCCG C^GGCCG
EclXI 13 CGGCCG C^GGCCG
Eco52I 13 CGGCCG C^GGCCG
Eco81I 790 CCTGAGG CC^T[ACGT]AGG
EcoRII 42 CCTGG ^CC[AT]GG
HincII 510 GTTAAC GT[CT]^[GA]AC
HindII 510 GTTAAC GT[CT]^[GA]AC
HpaI 510 GTTAAC GTT^AAC
HphI 317 TCACC GGTGA(8/7)
MaeI 538 CTAG C^TAG
MaeII 92 ACGT A^CGT
MslI 87 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 42 CCTGG CC^[AT]GG
NaeI 15 GCCGGC GCC^GGC
NdeI 542 CATATG CA^TATG
NgoMIV 15 GCCGGC G^CCGGC
PacI 478 TTAATTAA TTAAT^TAA
PpuMI 754 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 124 ATTAAT AT^TAAT
PvuII 499 CAGCTG CAG^CTG
RsaI 142 GTAC GT^AC
SfaNI 463 GATGC GCATC(5/9)
SfcI 427 CTACAG C^T[GA][CT]AG
SmaI 247 CCCGGG CCC^GGG
SspBI 141 TGTACA T^GTACA
StuI 575 AGGCCT AGG^CCT
TfiI 335 GAATC G^A[AT]TC
Tsp45I 547 GTGAC ^GT[GC]AC
TspRI 662 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 32 CCAGTCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 247 CCCGGG C^CCGGG
XmaI 247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 12 GCGG CCGC(-3/-1)
300 GCGG
AlwI 228 GGATC GGATC(4/5)
382 GGATC
BcnI 247 CCCGG CC^[GC]GG
248 CCGGG
BsaI 80 GAGACC GGTCTC(1/5)
180 GAGACC
BsaJI 247 CCCGGG C^C[ACGT][ACGT]GG
248 CCGGGG
BseRI 37 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsrDI 597 CATTGC GCAATG(2/0)
454 GCAATG
Cac8I 15 GCCGGC GC[ACGT]^[ACGT]GC
101 GCCTGC
EarI 759 CTCTTC CTCTTC(1/4)
177 GAAGAG
HapII 16 CCGG C^CGG
248 CCGG
HpaII 16 CCGG C^CGG
248 CCGG
Ksp632I 759 CTCTTC CTCTTC(1/4)
177 GAAGAG
MspA1I 10 CAGCGG C[AC]G^C[GT]G
499 CAGCTG
MspI 16 CCGG C^CGG
248 CCGG
MwoI 68 GCCGTCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
601 GCAGTTAAAGC
NciI 247 CCCGG CC^[GC]GG
248 CCGGG
NlaIII 156 CATG CATG^
583 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AspHI AvaII BbvI BmyI
BsiHKAI BsmAI Bsp1286I BsrI BssKI
BstF5I BstYI Cfr13I DdeI DpnII
DraII EcoO109I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp509I TthHB8I XhoII