Restriction map for
pF1KB7572
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AcsI AflII AflIII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbsI
BciVI BfrI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstZ17I
ClaI CpoI DraI DrdI DsaI
Eam1105I EarI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FokI FseI FspI
HindIII HinfI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 337 GTCTAC GT^[AC][GT]AC
AccIII 715 TCCGGA T^CCGGA
AclI 493 AACGTT AA^CGTT
Alw44I 679 GTGCAC G^TGCAC
AlwNI 77 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 10 CCTCAGG CC^T[ACGT]AGG
Aor51HI 476 AGCGCT AGC^GCT
ApaLI 679 GTGCAC G^TGCAC
AvaI 658 CCCGGG C^[CT]CG[GA]G
BamHI 712 GGATCC G^GATCC
BcgI 736 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 185 TGATCA T^GATCA
BlpI 555 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 555 GCTGAGC GC^T[ACGT]AGC
BsaI 393 GAGACC GGTCTC(1/5)
BseAI 715 TCCGGA T^CCGGA
BsgI 304 CTGCAC GTGCAG(16/14)
BsmAI 393 GAGAC GTCTC(1/5)
BsmFI 688 GTCCC GGGAC(10/14)
BsoBI 658 CCCGGG C^[CT]CG[GA]G
BspEI 715 TCCGGA T^CCGGA
BspMI 120 GCAGGT ACCTGC(4/8)
BsrBI 788 GAGCGG CCGCTC(-3/-3)
BsrI 181 CCAGT ACTGG(1/-1)
BssHII 739 GCGCGC G^CGCGC
BstYI 712 GGATCC [GA]^GATC[CT]
Bsu36I 10 CCTCAGG CC^T[ACGT]AGG
CelII 555 GCTGAGC GC^T[ACGT]AGC
DraIII 170 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 476 AGCGCT AGC^GCT
Eco81I 10 CCTCAGG CC^T[ACGT]AGG
EcoNI 417 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 185 TGATCA T^GATCA
HincII 496 GTTAAC GT[CT]^[GA]AC
HindII 496 GTTAAC GT[CT]^[GA]AC
HpaI 496 GTTAAC GTT^AAC
MaeII 494 ACGT A^CGT
MboII 368 TCTTC GAAGA(8/7)
MflI 712 GGATCC [GA]^GATC[CT]
MroI 715 TCCGGA T^CCGGA
MseI 497 TTAA T^TAA
MslI 801 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 256 CCGCTG C[AC]G^C[GT]G
PflMI 438 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 493 AACGTT AA^CGTT
SexAI 171 ACCTGGT A^CC[AT]GGT
SfaNI 365 GCATC GCATC(5/9)
SmaI 658 CCCGGG CCC^GGG
Tru9I 497 TTAA T^TAA
Van91I 438 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 227 CCACCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 712 GGATCC [GA]^GATC[CT]
XmaCI 658 CCCGGG C^CCGGG
XmaI 658 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 502 GGCGCC G[GA]^CG[CT]C
700 GGCGCC
AfaI 178 GTAC GT^AC
591 GTAC
AlwI 713 GATCC GGATC(4/5)
712 GGATC
ApaI 30 GGGCCC GGGCC^C
112 GGGCCC
AspHI 571 GTGCTC G[AT]GC[AT]^C
679 GTGCAC
BbeI 502 GGCGCC GGCGC^C
700 GGCGCC
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
516 GCTGAGG
BfaI 374 CTAG C^TAG
580 CTAG
BglI 156 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
727 GCCGAGGCGGC
BsaHI 502 GGCGCC G[GA]^CG[CT]C
700 GGCGCC
BsaWI 548 ACCGGA [AT]^CCGG[AT]
715 TCCGGA
BsiHKAI 571 GTGCTC G[AT]GC[AT]^C
679 GTGCAC
BsrFI 380 GCCGGT [GA]^CCGG[CT]
675 GCCGGT
BstXI 230 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
800 CCATCAAGATGG
Cfr10I 380 GCCGGT [GA]^CCGG[CT]
675 GCCGGT
DpnII 186 GATC ^GATC
713 GATC
EagI 673 CGGCCG C^GGCCG
761 CGGCCG
EclXI 673 CGGCCG C^GGCCG
761 CGGCCG
Eco52I 673 CGGCCG C^GGCCG
761 CGGCCG
HgaI 534 GACGC GACGC(5/10)
719 GACGC
Hin1I 502 GGCGCC G[GA]^CG[CT]C
700 GGCGCC
HphI 318 TCACC GGTGA(8/7)
461 TCACC
KasI 502 GGCGCC G^GCGCC
700 GGCGCC
MaeI 374 CTAG C^TAG
580 CTAG
MboI 186 GATC ^GATC
713 GATC
NarI 502 GGCGCC GG^CGCC
700 GGCGCC
NdeII 186 GATC ^GATC
713 GATC
PpuMI 354 AGGACCT [GA]G^G[AT]CC[CT]
686 GGGTCCC
RsaI 178 GTAC GT^AC
591 GTAC
Sau3AI 186 GATC ^GATC
713 GATC
SfoI 502 GGCGCC GGC^GCC
700 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI TspRI