Restriction map for
pF1KB7546
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MaeIII MluI MluNI MscI
MseI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 5 CGCG CG^CG
AcyI 195 GGCGCC G[GA]^CG[CT]C
AfaI 705 GTAC GT^AC
ApaI 671 GGGCCC GGGCC^C
AspHI 722 GTGCTC G[AT]GC[AT]^C
AspI 184 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 671 GGGCCC G[GA]GC[CT]^C
BbeI 195 GGCGCC GGCGC^C
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BcgI 369 CGAGGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 569 CTAG C^TAG
BsaBI 510 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 195 GGCGCC G[GA]^CG[CT]C
BseRI 339 CTCCTC GAGGAG(10/8)
BsiHKAI 722 GTGCTC G[AT]GC[AT]^C
BsmAI 352 GTCTC GTCTC(1/5)
BsrBI 657 CCGCTC CCGCTC(-3/-3)
BsrFI 321 ACCGGC [GA]^CCGG[CT]
BstAPI 467 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5 CGCG CG^CG
Cfr10I 321 ACCGGC [GA]^CCGG[CT]
DraII 571 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 264 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 354 CTCTTC CTCTTC(1/4)
EcoNI 563 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 571 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 195 GGCGCC [GA]GCGC^[CT]
HgaI 539 GCGTC GACGC(5/10)
Hin1I 195 GGCGCC G[GA]^CG[CT]C
HphI 319 TCACC GGTGA(8/7)
KasI 195 GGCGCC G^GCGCC
Ksp632I 354 CTCTTC CTCTTC(1/4)
MaeI 569 CTAG C^TAG
MamI 510 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 355 TCTTC GAAGA(8/7)
MfeI 212 CAATTG C^AATTG
MslI 688 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 412 CAGCTG C[AC]G^C[GT]G
MunI 212 CAATTG C^AATTG
MvnI 5 CGCG CG^CG
NarI 195 GGCGCC GG^CGCC
NlaIII 592 CATG CATG^
PflFI 184 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 114 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 412 CAGCTG CAG^CTG
RsaI 705 GTAC GT^AC
SfaNI 510 GATGC GCATC(5/9)
SfoI 195 GGCGCC GGC^GCC
SmlI 505 CTCAAG C^T[CT][GA]AG
TaqI 450 TCGA T^CGA
Tsp509I 213 AATT ^AATT
Tth111I 184 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 450 TCGA T^CGA
Van91I 114 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 169 CCAATGCTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 138 TCCGGA T^CCGGA
596 TCCGGA
AlwI 144 GATCC GGATC(4/5)
438 GGATC
Asp700I 558 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
615 GAAGCCCTTC
AvaII 599 GGACC G^G[AT]CC
728 GGACC
BanI 195 GGCGCC G^G[CT][GA]CC
499 GGTGCC
BsaWI 138 TCCGGA [AT]^CCGG[AT]
596 TCCGGA
BseAI 138 TCCGGA T^CCGGA
596 TCCGGA
BspEI 138 TCCGGA T^CCGGA
596 TCCGGA
BsrI 607 ACTGG ACTGG(1/-1)
708 CCAGT
BstXI 211 CCAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
407 CCAAGCAGCTGG
BstYI 143 AGATCC [GA]^GATC[CT]
438 GGATCT
CfoI 6 GCGC GCG^C
196 GCGC
DpnII 144 GATC ^GATC
439 GATC
EcoT14I 359 CCTTGG C^C[AT][AT]GG
434 CCAAGG
HhaI 6 GCGC GCG^C
196 GCGC
HinP1I 6 GCGC G^CGC
196 GCGC
MboI 144 GATC ^GATC
439 GATC
MflI 143 AGATCC [GA]^GATC[CT]
438 GGATCT
MroI 138 TCCGGA T^CCGGA
596 TCCGGA
NdeII 144 GATC ^GATC
439 GATC
PstI 64 CTGCAG CTGCA^G
636 CTGCAG
Sau3AI 144 GATC ^GATC
439 GATC
StyI 359 CCTTGG C^C[AT][AT]GG
434 CCAAGG
XhoII 143 AGATCC [GA]^GATC[CT]
438 GGATCT
XmnI 558 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
615 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MnlI MspI MvaI MwoI
NlaIV PleI Sau96I ScrFI SfcI
StuI TseI TspRI