Restriction map for
pF1KB7527
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstYI
BstZ17I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HhaI HinP1I HpaI HphI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MscI MslI MunI MvaI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
Sau96I SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TseI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 76 CGCG CG^CG
AccIII 104 TCCGGA T^CCGGA
AcyI 3 GGCGTC G[GA]^CG[CT]C
AocI 327 CCTCAGG CC^T[ACGT]AGG
BmyI 79 GTGCCC G[AGT]GC[ACT]^C
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaWI 104 TCCGGA [AT]^CCGG[AT]
BseAI 104 TCCGGA T^CCGGA
BseRI 457 GAGGAG GAGGAG(10/8)
Bsp1286I 79 GTGCCC G[AGT]GC[ACT]^C
BspEI 104 TCCGGA T^CCGGA
BspHI 377 TCATGA T^CATGA
BstF5I 446 CATCC GGATG(2/0)
BstUI 76 CGCG CG^CG
Bsu36I 327 CCTCAGG CC^T[ACGT]AGG
DdeI 328 CTCAG C^T[ACGT]AG
DraI 273 TTTAAA TTT^AAA
EarI 167 CTCTTC CTCTTC(1/4)
Eco81I 327 CCTCAGG CC^T[ACGT]AGG
EcoNI 348 CCTCGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 74 GCCGC GC^[ACGT]GC
FokI 446 CATCC GGATG(9/13)
HapII 105 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 343 GTCAAC GT[CT]^[GA]AC
HindII 343 GTCAAC GT[CT]^[GA]AC
HindIII 306 AAGCTT A^AGCTT
HpaII 105 CCGG C^CGG
ItaI 74 GCCGC GC^[ACGT]GC
Ksp632I 167 CTCTTC CTCTTC(1/4)
MroI 104 TCCGGA T^CCGGA
MspA1I 60 CCGCTG C[AC]G^C[GT]G
MspI 105 CCGG C^CGG
MvnI 76 CGCG CG^CG
MwoI 32 GCGTGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 441 GGAGCC GG[ACGT]^[ACGT]CC
NspI 389 ACATGC [GA]CATG^[CT]
RcaI 377 TCATGA T^CATGA
SapI 166 GCTCTTC GCTCTTC(1/4)
SfcI 218 CTATAG C^T[GA][CT]AG
TaqI 350 TCGA T^CGA
TfiI 289 GAATC G^A[AT]TC
TspRI 121 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 350 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 60 CCGC CCGC(-3/-1)
75 CCGC
AcsI 270 GAATTT [GA]^AATT[CT]
279 GAATTT
ApoI 270 GAATTT [GA]^AATT[CT]
279 GAATTT
BbsI 250 GAAGAC GAAGAC(2/6)
361 GAAGAC
BpuAI 250 GAAGAC GAAGAC(2/6)
361 GAAGAC
BsiYI 348 CCTCGATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
450 CCAGAATGAGG
BslI 348 CCTCGATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
450 CCAGAATGAGG
Cac8I 70 GCCTGC GC[ACGT]^[ACGT]GC
77 GCGTGC
MseI 274 TTAA T^TAA
301 TTAA
NlaIII 378 CATG CATG^
390 CATG
PleI 214 GACTC GAGTC(4/5)
460 GAGTC
Tru9I 274 TTAA T^TAA
301 TTAA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI DpnII HinfI MboI MboII
MnlI NdeII Sau3AI Tsp509I