Restriction map for
pF1KB7452
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaII AvrII
BalI BamHI BbrPI BbvCI BcgI
BclI BfaI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
HincII HindII HindIII HpaI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 30 GGTACC G^GTACC
AfaI 31 GTAC GT^AC
Asp718I 30 GGTACC G^GTACC
AviII 494 TGCGCA TGC^GCA
BanII 256 GAGCCC G[GA]GC[CT]^C
BbsI 538 GTCTTC GAAGAC(2/6)
BciVI 73 GTATCC GTATCC(6/5)
BlpI 531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 531 GCTGAGC GC^T[ACGT]AGC
BpuAI 538 GTCTTC GAAGAC(2/6)
BsaAI 143 TACGTG [CT]AC^GT[GA]
BsgI 502 GTGCAG GTGCAG(16/14)
BsmFI 455 GGGAC GGGAC(10/14)
BsrI 66 CCAGT ACTGG(1/-1)
BssSI 517 CACGAG CACGAG(-5/-1)
CelII 531 GCTGAGC GC^T[ACGT]AGC
DpnII 189 GATC ^GATC
DsaI 632 CCGTGG C^C[GA][CT]GG
FspI 494 TGCGCA TGC^GCA
HinfI 619 GACTC G^A[ACGT]TC
HphI 557 TCACC GGTGA(8/7)
KpnI 30 GGTACC GGTAC^C
MboI 189 GATC ^GATC
NdeII 189 GATC ^GATC
NotI 59 GCGGCCGC GC^GGCCGC
NruI 791 TCGCGA TCG^CGA
PflMI 606 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 619 GACTC GAGTC(4/5)
PvuI 188 CGATCG CGAT^CG
PvuII 607 CAGCTG CAG^CTG
RsaI 31 GTAC GT^AC
Sau3AI 189 GATC ^GATC
SfaNI 446 GCATC GCATC(5/9)
TaqI 212 TCGA T^CGA
TthHB8I 212 TCGA T^CGA
Van91I 606 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BmyI 256 GAGCCC G[AGT]GC[ACT]^C
549 GGGCAC
Bsp1286I 256 GAGCCC G[AGT]GC[ACT]^C
549 GGGCAC
BspMI 559 ACCTGC ACCTGC(4/8)
120 GCAGGT
EcoNI 114 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
153 CCTGCTGAAGG
HgaI 697 GACGC GACGC(5/10)
536 GCGTC
MaeII 144 ACGT A^CGT
356 ACGT
MaeIII 130 GTGAC ^GT[ACGT]AC
512 GTGAC
NlaIII 219 CATG CATG^
747 CATG
PstI 277 CTGCAG CTGCA^G
685 CTGCAG
SapI 600 GCTCTTC GCTCTTC(1/4)
783 GCTCTTC
SgrAI 384 CGCCGGCG C[GA]^CCGG[CT]G
591 CGCCGGCG
SmaI 77 CCCGGG CCC^GGG
261 CCCGGG
Tsp45I 130 GTGAC ^GT[GC]AC
512 GTGAC
TspRI 8 CAGTG CA[GC]TG[ACGT][ACGT]^
505 CAGTG
XmaCI 77 CCCGGG C^CCGGG
261 CCCGGG
XmaI 77 CCCGGG C^CCGGG
261 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI BanI BbeI BbvI BcnI
BglI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsoBI BsrFI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI EaeI
EagI EarI EclXI Eco52I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI Ksp632I MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIV Sau96I
ScrFI SfcI SfoI TseI