Restriction map for
pF1KB7448
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BspDI BspEI BspMI BsrBI BsrFI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
Ksp632I KspI MamI MflI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 243 GGTACC G^GTACC
AcsI 413 GAATTC [GA]^AATT[CT]
AflIII 730 ACATGT A^C[GA][CT]GT
ApoI 413 GAATTC [GA]^AATT[CT]
Asp718I 243 GGTACC G^GTACC
AvaI 270 CTCGGG C^[CT]CG[GA]G
BalI 563 TGGCCA TGG^CCA
BanI 243 GGTACC G^G[CT][GA]CC
BciVI 59 GTATCC GTATCC(6/5)
BclI 857 TGATCA T^GATCA
BfaI 841 CTAG C^TAG
BpmI 913 CTGGAG CTGGAG(16/14)
BsaAI 853 TACGTG [CT]AC^GT[GA]
BsmBI 343 GAGACG CGTCTC(1/5)
BsoBI 270 CTCGGG C^[CT]CG[GA]G
Bsp1407I 113 TGTACA T^GTACA
BspHI 476 TCATGA T^CATGA
BspLU11I 730 ACATGT A^CATGT
BsrDI 651 CATTGC GCAATG(2/0)
BsrGI 113 TGTACA T^GTACA
BstAPI 260 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 873 TTTAAA TTT^AAA
DraIII 101 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 413 GAATTC G^AATTC
EcoT14I 362 CCATGG C^C[AT][AT]GG
FbaI 857 TGATCA T^GATCA
KpnI 243 GGTACC GGTAC^C
MaeI 841 CTAG C^TAG
MaeII 854 ACGT A^CGT
MfeI 907 CAATTG C^AATTG
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
MspA1I 335 CAGCTG C[AC]G^C[GT]G
MunI 907 CAATTG C^AATTG
NcoI 362 CCATGG C^CATGG
NheI 840 GCTAGC G^CTAGC
PflMI 21 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 480 GACTC GAGTC(4/5)
PvuII 335 CAGCTG CAG^CTG
RcaI 476 TCATGA T^CATGA
SfcI 603 CTACAG C^T[GA][CT]AG
SmlI 425 CTCAAG C^T[CT][GA]AG
SspBI 113 TGTACA T^GTACA
StyI 362 CCATGG C^C[AT][AT]GG
Van91I 21 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 902 CCAAACAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 375 CCGC CCGC(-3/-1)
793 GCGG
AvaII 7 GGTCC G^G[AT]CC
899 GGACC
BanII 181 GAGCCC G[GA]GC[CT]^C
633 GGGCTC
BseRI 234 CTCCTC GAGGAG(10/8)
696 CTCCTC
BstXI 332 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
484 CCACTGAGTTGG
Cfr13I 7 GGTCC G^G[ACGT]CC
899 GGACC
DpnII 691 GATC ^GATC
858 GATC
DsaI 239 CCGTGG C^C[GA][CT]GG
362 CCATGG
EaeI 449 TGGCCG [CT]^GGCC[GA]
563 TGGCCA
HphI 255 GGTGA GGTGA(8/7)
455 TCACC
MboI 691 GATC ^GATC
858 GATC
MboII 76 TCTTC GAAGA(8/7)
263 TCTTC
NdeII 691 GATC ^GATC
858 GATC
NspI 574 GCATGT [GA]CATG^[CT]
730 ACATGT
Sau3AI 691 GATC ^GATC
858 GATC
Sau96I 7 GGTCC G^G[ACGT]CC
899 GGACC
TfiI 166 GATTC G^A[AT]TC
501 GAATC
TspRI 101 CACTG CA[GC]TG[ACGT][ACGT]^
485 CACTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MnlI MseI MslI MvaI
MwoI NlaIII NlaIV RsaI ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I