Restriction map for
pF1KB7434
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 13 GACGCC G[GA]^CG[CT]C
AfaI 437 GTAC GT^AC
AocI 547 CCTGAGG CC^T[ACGT]AGG
AvaI 423 CCCGGG C^[CT]CG[GA]G
BfaI 181 CTAG C^TAG
BmyI 417 GTGCCC G[AGT]GC[ACT]^C
BpmI 310 CTCCAG CTGGAG(16/14)
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 27 GAGACC GGTCTC(1/5)
BsiEI 258 CGATCG CG[GA][CT]^CG
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 423 CCCGGG C^[CT]CG[GA]G
Bsp1286I 417 GTGCCC G[AGT]GC[ACT]^C
BspHI 170 TCATGA T^CATGA
BstEII 192 GGTCACC G^GT[ACGT]ACC
BstF5I 36 GGATG GGATG(2/0)
BstPI 192 GGTCACC G^GT[ACGT]ACC
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
Cac8I 345 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 237 CCACGG C^C[GA][CT]GG
EarI 532 GAAGAG CTCTTC(1/4)
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
EcoNI 17 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
EcoT14I 440 CCTTGG C^C[AT][AT]GG
FokI 36 GGATG GGATG(9/13)
HapII 424 CCGG C^CGG
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HpaII 424 CCGG C^CGG
HphI 194 TCACC GGTGA(8/7)
Ksp632I 532 GAAGAG CTCTTC(1/4)
MaeI 181 CTAG C^TAG
MaeII 285 ACGT A^CGT
MseI 452 TTAA T^TAA
MslI 238 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 424 CCGG C^CGG
MwoI 336 GCCCAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 483 ACATGC [GA]CATG^[CT]
PstI 227 CTGCAG CTGCA^G
PvuI 258 CGATCG CGAT^CG
RcaI 170 TCATGA T^CATGA
RsaI 437 GTAC GT^AC
SfcI 227 CTGCAG C^T[GA][CT]AG
SmaI 423 CCCGGG CCC^GGG
StuI 68 AGGCCT AGG^CCT
StyI 440 CCTTGG C^C[AT][AT]GG
Tru9I 452 TTAA T^TAA
Tsp45I 193 GTCAC ^GT[GC]AC
XmaCI 423 CCCGGG C^CCGGG
XmaI 423 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaII 466 GGACC G^G[AT]CC
491 GGACC
BcnI 423 CCCGG CC^[GC]GG
424 CCGGG
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
BstNI 32 CCAGG CC^[AT]GG
488 CCTGG
EcoRII 32 CCAGG ^CC[AT]GG
488 CCTGG
HaeIII 69 GGCC GG^CC
391 GGCC
HinfI 273 GAATC G^A[ACGT]TC
317 GAATC
MspA1I 412 CAGCTG C[AC]G^C[GT]G
517 CAGCTG
MvaI 32 CCAGG CC^[AT]GG
488 CCTGG
NciI 423 CCCGG CC^[GC]GG
424 CCGGG
NlaIV 466 GGACCC GG[ACGT]^[ACGT]CC
491 GGACCC
PvuII 412 CAGCTG CAG^CTG
517 CAGCTG
SfaNI 40 GATGC GCATC(5/9)
385 GCATC
TaqI 74 TCGA T^CGA
261 TCGA
TfiI 273 GAATC G^A[AT]TC
317 GAATC
TthHB8I 74 TCGA T^CGA
261 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BcgI BsaJI BsiYI
BslI BssKI Cfr13I DdeI DpnII
Fnu4HI ItaI MaeIII MboI MboII
MnlI NdeII NlaIII Sau3AI Sau96I
ScrFI TseI