Restriction map for
pF1KB7430
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MaeII MaeIII MamI MflI
MluI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 754 GTAGAC GT^[AC][GT]AC
AccIII 21 TCCGGA T^CCGGA
AfaI 377 GTAC GT^AC
AlwI 1030 GGATC GGATC(4/5)
Aor51HI 322 AGCGCT AGC^GCT
BalI 504 TGGCCA TGG^CCA
BbsI 985 GAAGAC GAAGAC(2/6)
BbvCI 1479 CCTCAGC CCTCAGC(-5/-2)
BglI 506 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1279 CTCCAG CTGGAG(16/14)
BpuAI 985 GAAGAC GAAGAC(2/6)
BsaWI 21 TCCGGA [AT]^CCGG[AT]
BseAI 21 TCCGGA T^CCGGA
BspDI 1132 ATCGAT AT^CGAT
BspEI 21 TCCGGA T^CCGGA
BsrFI 70 GCCGGC [GA]^CCGG[CT]
BstAPI 515 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
ClaI 1132 ATCGAT AT^CGAT
EagI 33 CGGCCG C^GGCCG
EclXI 33 CGGCCG C^GGCCG
Eco47III 322 AGCGCT AGC^GCT
Eco52I 33 CGGCCG C^GGCCG
EcoNI 922 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 964 CCTTGG C^C[AT][AT]GG
FauI 56 CCCGC CCCGC(4/6)
HindIII 1339 AAGCTT A^AGCTT
HphI 1051 TCACC GGTGA(8/7)
MfeI 593 CAATTG C^AATTG
MluNI 504 TGGCCA TGG^CCA
MroI 21 TCCGGA T^CCGGA
MscI 504 TGGCCA TGG^CCA
MunI 593 CAATTG C^AATTG
NaeI 70 GCCGGC GCC^GGC
NgoMIV 70 GCCGGC G^CCGGC
NspI 1200 ACATGC [GA]CATG^[CT]
PvuI 1134 CGATCG CGAT^CG
PvuII 907 CAGCTG CAG^CTG
RsaI 377 GTAC GT^AC
SspI 827 AATATT AAT^ATT
StyI 964 CCTTGG C^C[AT][AT]GG
XcmI 454 CCACTTCGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 691 AAATTT [GA]^AATT[CT]
1327 GAATTT
ApoI 691 AAATTT [GA]^AATT[CT]
1327 GAATTT
BanII 53 GAGCCC G[GA]GC[CT]^C
1193 GGGCTC
BmyI 53 GAGCCC G[AGT]GC[ACT]^C
1193 GGGCTC
BsiEI 33 CGGCCG CG[GA][CT]^CG
1134 CGATCG
Bsp1286I 53 GAGCCC G[AGT]GC[ACT]^C
1193 GGGCTC
BsrI 196 ACTGG ACTGG(1/-1)
1027 ACTGG
CfoI 9 GCGC GCG^C
323 GCGC
Cfr13I 73 GGCCC G^G[ACGT]CC
598 GGGCC
HaeII 8 GGCGCT [GA]GCGC^[CT]
322 AGCGCT
HhaI 9 GCGC GCG^C
323 GCGC
HinP1I 9 GCGC G^CGC
323 GCGC
HincII 842 GTCAAC GT[CT]^[GA]AC
1466 GTCAAC
HindII 842 GTCAAC GT[CT]^[GA]AC
1466 GTCAAC
MslI 86 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1159 CACCTGCATG
MspA1I 30 CAGCGG C[AC]G^C[GT]G
907 CAGCTG
NlaIV 18 GGCTCC GG[ACGT]^[ACGT]CC
52 GGAGCC
Sau96I 73 GGCCC G^G[ACGT]CC
598 GGGCC
SfcI 702 CTGTAG C^T[GA][CT]AG
752 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI Asp700I BbvI BcnI
BfaI BsaJI BsiYI BslI BsmAI
BspMI BssKI BstNI Cac8I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII PleI Sau3AI ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI