Restriction map for
pF1KB7415
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 546 GTAC GT^AC
AlwNI 164 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 812 GAGCTC G[AT]GC[AT]^C
AvaI 664 CCCGAG C^[CT]CG[GA]G
BanII 812 GAGCTC G[GA]GC[CT]^C
BbsI 577 GTCTTC GAAGAC(2/6)
BbvI 159 GCAGC GCAGC(8/12)
BpmI 143 CTCCAG CTGGAG(16/14)
BpuAI 577 GTCTTC GAAGAC(2/6)
BseRI 376 GAGGAG GAGGAG(10/8)
BsiHKAI 812 GAGCTC G[AT]GC[AT]^C
BsmBI 8 CGTCTC CGTCTC(1/5)
BsmI 388 GCATTC GAATGC(1/-1)
BsoBI 664 CCCGAG C^[CT]CG[GA]G
BspHI 108 TCATGA T^CATGA
BstAPI 627 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 290 TTCGAA TT^CGAA
DraI 540 TTTAAA TTT^AAA
DraII 150 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 815 CTCTTC CTCTTC(1/4)
EcoO109I 150 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 272 GAATTC G^AATTC
EcoT22I 386 ATGCAT ATGCA^T
Fnu4HI 159 GCAGC GC^[ACGT]GC
HapII 58 CCGG C^CGG
HinfI 112 GATTC G^A[ACGT]TC
HpaII 58 CCGG C^CGG
ItaI 159 GCAGC GC^[ACGT]GC
Ksp632I 815 CTCTTC CTCTTC(1/4)
MseI 541 TTAA T^TAA
MslI 436 CACTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 58 CCGG C^CGG
NheI 757 GCTAGC G^CTAGC
NlaIV 836 GGGTCC GG[ACGT]^[ACGT]CC
NsiI 386 ATGCAT ATGCA^T
NspI 65 GCATGC [GA]CATG^[CT]
NspV 290 TTCGAA TT^CGAA
PpuMI 150 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 108 TCATGA T^CATGA
RsaI 546 GTAC GT^AC
SacI 812 GAGCTC GAGCT^C
SapI 814 GCTCTTC GCTCTTC(1/4)
SfuI 290 TTCGAA TT^CGAA
SmlI 373 CTTGAG C^T[CT][GA]AG
SphI 65 GCATGC GCATG^C
SspI 871 AATATT AAT^ATT
StuI 369 AGGCCT AGG^CCT
TaqI 291 TCGA T^CGA
TfiI 112 GATTC G^A[AT]TC
Tru9I 541 TTAA T^TAA
TseI 159 GCAGC G^C[AT]GC
TthHB8I 291 TCGA T^CGA
XcmI 258 CCAGCTAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 840 CCGC CCGC(-3/-1)
219 GCGG
AlwI 675 GATCC GGATC(4/5)
718 GGATC
BalI 203 TGGCCA TGG^CCA
255 TGGCCA
BfaI 262 CTAG C^TAG
758 CTAG
BsaI 311 GAGACC GGTCTC(1/5)
104 GGTCTC
BstXI 435 CCACTTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
637 CCAGCACCCTGG
EaeI 203 TGGCCA [CT]^GGCC[GA]
255 TGGCCA
MaeI 262 CTAG C^TAG
758 CTAG
MaeIII 877 GTCAC ^GT[ACGT]AC
902 GTGAC
MluNI 203 TGGCCA TGG^CCA
255 TGGCCA
MscI 203 TGGCCA TGG^CCA
255 TGGCCA
Tsp45I 877 GTCAC ^GT[GC]AC
902 GTGAC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI ApoI AvaII BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HphI MboI MboII MnlI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI Tsp509I TspRI