Restriction map for
pF1KB7412
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeIII MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 841 GACGTC GACGT^C
BalI 197 TGGCCA TGG^CCA
BamHI 786 GGATCC G^GATCC
BanII 359 GGGCTC G[GA]GC[CT]^C
BbsI 776 GTCTTC GAAGAC(2/6)
BciVI 761 GTATCC GTATCC(6/5)
BfaI 233 CTAG C^TAG
BpuAI 776 GTCTTC GAAGAC(2/6)
BseRI 64 CTCCTC GAGGAG(10/8)
BsmAI 374 GAGAC GTCTC(1/5)
BsrFI 28 GCCGGT [GA]^CCGG[CT]
BssHII 209 GCGCGC G^CGCGC
BstXI 659 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 786 GGATCC [GA]^GATC[CT]
Cfr10I 28 GCCGGT [GA]^CCGG[CT]
DraII 460 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 203 CCGCGG C^C[GA][CT]GG
EaeI 197 TGGCCA [CT]^GGCC[GA]
EcoNI 522 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 460 GGGTCCT [GA]G^G[ACGT]CC[CT]
HphI 687 GGTGA GGTGA(8/7)
KspI 203 CCGCGG CCGC^GG
MaeI 233 CTAG C^TAG
MflI 786 GGATCC [GA]^GATC[CT]
MluNI 197 TGGCCA TGG^CCA
MscI 197 TGGCCA TGG^CCA
MseI 562 TTAA T^TAA
MslI 25 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 232 GCTAGC G^CTAGC
NspI 509 GCATGC [GA]CATG^[CT]
PleI 532 GACTC GAGTC(4/5)
PpuMI 460 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 160 CTGCAG CTGCA^G
SacII 203 CCGCGG CCGC^GG
SapI 566 GAAGAGC GCTCTTC(1/4)
SfaNI 478 GCATC GCATC(5/9)
SfcI 160 CTGCAG C^T[GA][CT]AG
SgrAI 27 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 858 CTCAAG C^T[CT][GA]AG
SphI 509 GCATGC GCATG^C
TaqI 119 TCGA T^CGA
TfiI 679 GAATC G^A[AT]TC
Tru9I 562 TTAA T^TAA
TthHB8I 119 TCGA T^CGA
XhoII 786 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 787 GATCC GGATC(4/5)
786 GGATC
AspHI 319 GTGCTC G[AT]GC[AT]^C
426 GAGCAC
BpmI 884 CTCCAG CTGGAG(16/14)
601 CTGGAG
BsiEI 14 CGGTCG CG[GA][CT]^CG
188 CGACCG
BsiHKAI 319 GTGCTC G[AT]GC[AT]^C
426 GAGCAC
BstF5I 479 CATCC GGATG(2/0)
713 GGATG
DpnII 117 GATC ^GATC
787 GATC
EarI 502 GAAGAG CTCTTC(1/4)
566 GAAGAG
EcoRI 726 GAATTC G^AATTC
879 GAATTC
FokI 479 CATCC GGATG(9/13)
713 GGATG
HinfI 532 GACTC G^A[ACGT]TC
679 GAATC
Ksp632I 502 GAAGAG CTCTTC(1/4)
566 GAAGAG
MaeII 44 ACGT A^CGT
842 ACGT
MboI 117 GATC ^GATC
787 GATC
MspA1I 77 CCGCTG C[AC]G^C[GT]G
203 CCGCGG
NdeII 117 GATC ^GATC
787 GATC
NlaIII 510 CATG CATG^
824 CATG
Sau3AI 117 GATC ^GATC
787 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AvaII BanI BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MboII
MnlI MspI MvaI MvnI MwoI
NarI NciI NlaIV RsaI Sau96I
ScrFI SfoI TseI Tsp509I TspRI