Restriction map for
pF1KB7411
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HpaI KpnI
KspI MaeI MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 546 GTATAC GT^[AC][GT]AC
AciI 369 CCGC CCGC(-3/-1)
AcsI 166 AAATTT [GA]^AATT[CT]
AcyI 704 GGCGCC G[GA]^CG[CT]C
AocI 879 CCTAAGG CC^T[ACGT]AGG
ApoI 166 AAATTT [GA]^AATT[CT]
AspI 333 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 704 GGCGCC G^G[CT][GA]CC
BanII 31 GAGCCC G[GA]GC[CT]^C
BbeI 704 GGCGCC GGCGC^C
BsaHI 704 GGCGCC G[GA]^CG[CT]C
BsaWI 497 TCCGGT [AT]^CCGG[AT]
BsgI 318 CTGCAC GTGCAG(16/14)
BsmFI 763 GGGAC GGGAC(10/14)
Bsp1407I 631 TGTACA T^GTACA
BspMI 593 ACCTGC ACCTGC(4/8)
BsrGI 631 TGTACA T^GTACA
BssKI 725 CCTGG ^CC[ACGT]GG
Bst1107I 546 GTATAC GTA^TAC
BstNI 725 CCTGG CC^[AT]GG
BstZ17I 546 GTATAC GTA^TAC
Bsu36I 879 CCTAAGG CC^T[ACGT]AGG
Cac8I 348 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 705 GCGC GCG^C
DsaI 759 CCATGG C^C[GA][CT]GG
Eco81I 879 CCTAAGG CC^T[ACGT]AGG
EcoRII 725 CCTGG ^CC[AT]GG
HaeII 704 GGCGCC [GA]GCGC^[CT]
HapII 498 CCGG C^CGG
HhaI 705 GCGC GCG^C
Hin1I 704 GGCGCC G[GA]^CG[CT]C
HinP1I 705 GCGC G^CGC
HincII 847 GTCAAC GT[CT]^[GA]AC
HindII 847 GTCAAC GT[CT]^[GA]AC
HindIII 70 AAGCTT A^AGCTT
HpaII 498 CCGG C^CGG
KasI 704 GGCGCC G^GCGCC
MaeII 893 ACGT A^CGT
MspI 498 CCGG C^CGG
MvaI 725 CCTGG CC^[AT]GG
NarI 704 GGCGCC GG^CGCC
NcoI 759 CCATGG C^CATGG
NspI 461 GCATGT [GA]CATG^[CT]
PflFI 333 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 337 GAGTC GAGTC(4/5)
ScaI 305 AGTACT AGT^ACT
ScrFI 725 CCTGG CC^[ACGT]GG
SfcI 873 CTACAG C^T[GA][CT]AG
SfoI 704 GGCGCC GGC^GCC
SmlI 250 CTTGAG C^T[CT][GA]AG
SspBI 631 TGTACA T^GTACA
Tsp509I 167 AATT ^AATT
Tth111I 333 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 514 GGACC G^G[AT]CC
764 GGACC
BalI 377 TGGCCA TGG^CCA
407 TGGCCA
BclI 140 TGATCA T^GATCA
641 TGATCA
BmyI 31 GAGCCC G[AGT]GC[ACT]^C
428 GTGCCC
BsiYI 369 CCGCTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
432 CCTGCTGGTGG
BslI 369 CCGCTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
432 CCTGCTGGTGG
Bsp1286I 31 GAGCCC G[AGT]GC[ACT]^C
428 GTGCCC
EaeI 377 TGGCCA [CT]^GGCC[GA]
407 TGGCCA
EarI 85 CTCTTC CTCTTC(1/4)
225 CTCTTC
FbaI 140 TGATCA T^GATCA
641 TGATCA
Ksp632I 85 CTCTTC CTCTTC(1/4)
225 CTCTTC
MluNI 377 TGGCCA TGG^CCA
407 TGGCCA
MscI 377 TGGCCA TGG^CCA
407 TGGCCA
MwoI 352 GCAGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
461 GCATGTTTGGC
TfiI 18 GAATC G^A[AT]TC
78 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BstF5I
Cfr13I DdeI DpnII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I SfaNI StyI Tru9I
TseI Tsp45I TspRI