Restriction map for
pF1KB7410
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 502 AAATTC [GA]^AATT[CT]
AfaI 390 GTAC GT^AC
AflIII 646 ACATGT A^C[GA][CT]GT
AlwI 243 GGATC GGATC(4/5)
AlwNI 142 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 502 AAATTC [GA]^AATT[CT]
AvaI 9 CTCGGG C^[CT]CG[GA]G
BfaI 700 CTAG C^TAG
BglII 688 AGATCT A^GATCT
BpmI 247 CTCCAG CTGGAG(16/14)
BsaBI 603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 6 GGTCTC GGTCTC(1/5)
BsaWI 567 ACCGGA [AT]^CCGG[AT]
BsmI 933 GAATGC GAATGC(1/-1)
BsoBI 9 CTCGGG C^[CT]CG[GA]G
BspLU11I 646 ACATGT A^CATGT
BspMI 822 GCAGGT ACCTGC(4/8)
BssSI 753 CTCGTG CACGAG(-5/-1)
BstEII 90 GGTGACC G^GT[ACGT]ACC
BstPI 90 GGTGACC G^GT[ACGT]ACC
CfoI 697 GCGC GCG^C
DrdI 136 GACGGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 138 CGGCCA [CT]^GGCC[GA]
EcoO65I 90 GGTGACC G^GT[ACGT]ACC
EcoT14I 737 CCATGG C^C[AT][AT]GG
HapII 568 CCGG C^CGG
HhaI 697 GCGC GCG^C
HinP1I 697 GCGC G^CGC
HpaII 568 CCGG C^CGG
MaeI 700 CTAG C^TAG
MamI 603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 890 CAGCTG C[AC]G^C[GT]G
MspI 568 CCGG C^CGG
NcoI 737 CCATGG C^CATGG
PleI 779 GACTC GAGTC(4/5)
PpuMI 143 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 890 CAGCTG CAG^CTG
RsaI 390 GTAC GT^AC
ScaI 389 AGTACT AGT^ACT
SphI 398 GCATGC GCATG^C
StyI 737 CCATGG C^C[AT][AT]GG
TaqI 929 TCGA T^CGA
TfiI 200 GAATC G^A[AT]TC
Tsp509I 503 AATT ^AATT
TthHB8I 929 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 519 CCGC CCGC(-3/-1)
581 CCGC
Asp700I 619 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
933 GAATGCCTTC
AspHI 190 GAGCTC G[AT]GC[AT]^C
312 GAGCTC
BanI 14 GGCACC G^G[CT][GA]CC
539 GGTGCC
BanII 190 GAGCTC G[GA]GC[CT]^C
312 GAGCTC
BciVI 981 GGATAC GTATCC(6/5)
419 GTATCC
BsgI 330 CTGCAC GTGCAG(16/14)
887 GTGCAG
BsiHKAI 190 GAGCTC G[AT]GC[AT]^C
312 GAGCTC
BsmAI 913 GAGAC GTCTC(1/5)
7 GTCTC
BsmFI 595 GTCCC GGGAC(10/14)
758 GTCCC
BsrDI 855 CATTGC GCAATG(2/0)
1056 CATTGC
BsrI 299 ACTGG ACTGG(1/-1)
765 ACTGG
BstYI 243 GGATCT [GA]^GATC[CT]
688 AGATCT
DsaI 347 CCGTGG C^C[GA][CT]GG
737 CCATGG
FauI 518 CCCGC CCCGC(4/6)
580 CCCGC
HinfI 200 GAATC G^A[ACGT]TC
779 GACTC
MflI 243 GGATCT [GA]^GATC[CT]
688 AGATCT
MseI 284 TTAA T^TAA
1145 TTAA
NspI 398 GCATGC [GA]CATG^[CT]
646 ACATGT
PflMI 141 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
293 CCACACACTGG
SacI 190 GAGCTC GAGCT^C
312 GAGCTC
SapI 619 GAAGAGC GCTCTTC(1/4)
57 GCTCTTC
SfaNI 32 GATGC GCATC(5/9)
255 GCATC
Tru9I 284 TTAA T^TAA
1145 TTAA
Van91I 141 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
293 CCACACACTGG
XhoII 243 GGATCT [GA]^GATC[CT]
688 AGATCT
XmnI 619 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
933 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AvaII BbsI BbvI BmyI
BpuAI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI TseI Tsp45I TspRI