Restriction map for
pF1KB7270
##################################
# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AviII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstNI BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
ApaI 28 GGGCCC GGGCC^C
AvaI 24 CCCGGG C^[CT]CG[GA]G
AvrII 70 CCTAGG C^CTAGG
BlnI 70 CCTAGG C^CTAGG
BsgI 468 GTGCAG GTGCAG(16/14)
BsiYI 18 CCAGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 18 CCAGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 10 GAGAC GTCTC(1/5)
BsmFI 151 GGGAC GGGAC(10/14)
BsoBI 24 CCCGGG C^[CT]CG[GA]G
Bsp1407I 195 TGTACA T^GTACA
BsrGI 195 TGTACA T^GTACA
BstAPI 574 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 49 GGATG GGATG(2/0)
Cac8I 238 GCTGGC GC[ACGT]^[ACGT]GC
DraII 27 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 27 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 70 CCTAGG C^C[AT][AT]GG
FokI 49 GGATG GGATG(9/13)
HaeIII 29 GGCC GG^CC
HapII 25 CCGG C^CGG
HpaII 25 CCGG C^CGG
HphI 531 TCACC GGTGA(8/7)
MseI 217 TTAA T^TAA
MspI 25 CCGG C^CGG
PstI 581 CTGCAG CTGCA^G
ScaI 403 AGTACT AGT^ACT
SfaNI 190 GCATC GCATC(5/9)
SfcI 581 CTGCAG C^T[GA][CT]AG
SmaI 24 CCCGGG CCC^GGG
SspBI 195 TGTACA T^GTACA
StyI 70 CCTAGG C^C[AT][AT]GG
Tru9I 217 TTAA T^TAA
Tsp45I 370 GTCAC ^GT[GC]AC
XmaCI 24 CCCGGG C^CCGGG
XmaI 24 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 513 AAATTT [GA]^AATT[CT]
626 AAATTC
AfaI 196 GTAC GT^AC
404 GTAC
AlwNI 182 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
575 CAGAAGCTG
ApoI 513 AAATTT [GA]^AATT[CT]
626 AAATTC
BanII 28 GGGCCC G[GA]GC[CT]^C
141 GAGCCC
BcnI 24 CCCGG CC^[GC]GG
25 CCGGG
BmyI 28 GGGCCC G[AGT]GC[ACT]^C
141 GAGCCC
Bsp1286I 28 GGGCCC G[AGT]GC[ACT]^C
141 GAGCCC
BssKI 24 CCCGG ^CC[ACGT]GG
25 CCGGG
Cfr13I 28 GGGCC G^G[ACGT]CC
29 GGCCC
HinfI 184 GATTC G^A[ACGT]TC
603 GAATC
MboII 418 GAAGA GAAGA(8/7)
111 TCTTC
MwoI 134 GCAGAATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
574 GCAGAAGCTGC
NciI 24 CCCGG CC^[GC]GG
25 CCGGG
NlaIII 33 CATG CATG^
465 CATG
RsaI 196 GTAC GT^AC
404 GTAC
Sau96I 28 GGGCC G^G[ACGT]CC
29 GGCCC
ScrFI 24 CCCGG CC^[ACGT]GG
25 CCGGG
TfiI 184 GATTC G^A[AT]TC
603 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI Asp700I BbvI BfaI BsaJI
BsrI DdeI Fnu4HI ItaI MaeI
MaeIII MnlI NlaIV TseI Tsp509I
XmnI