Restriction map for
pF1KB7266
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BcgI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 994 GTCTAC GT^[AC][GT]AC
AccII 999 CGCG CG^CG
AciI 321 GCGG CCGC(-3/-1)
AlwNI 664 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 875 CCTCAGG CC^T[ACGT]AGG
BbvCI 812 CCTCAGC CCTCAGC(-5/-2)
BciVI 739 GTATCC GTATCC(6/5)
BcnI 475 CCGGG CC^[GC]GG
BmyI 477 GGGCAC G[AGT]GC[ACT]^C
BseRI 155 GAGGAG GAGGAG(10/8)
Bsp1286I 477 GGGCAC G[AGT]GC[ACT]^C
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BssSI 550 CACGAG CACGAG(-5/-1)
BstAPI 928 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 403 TTCGAA TT^CGAA
BstUI 999 CGCG CG^CG
Bsu36I 875 CCTCAGG CC^T[ACGT]AGG
CfoI 1000 GCGC GCG^C
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
EarI 674 GAAGAG CTCTTC(1/4)
Eco81I 875 CCTCAGG CC^T[ACGT]AGG
EcoT14I 971 CCAAGG C^C[AT][AT]GG
HhaI 1000 GCGC GCG^C
HinP1I 1000 GCGC G^CGC
Ksp632I 674 GAAGAG CTCTTC(1/4)
MaeII 398 ACGT A^CGT
MslI 550 CACGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 773 CAGCTG C[AC]G^C[GT]G
MvnI 999 CGCG CG^CG
NaeI 46 GCCGGC GCC^GGC
NciI 475 CCGGG CC^[GC]GG
NgoMIV 46 GCCGGC G^CCGGC
NlaIV 870 GGTTCC GG[ACGT]^[ACGT]CC
NspV 403 TTCGAA TT^CGAA
PstI 583 CTGCAG CTGCA^G
PvuII 773 CAGCTG CAG^CTG
SbfI 582 CCTGCAGG CCTGCA^GG
SfuI 403 TTCGAA TT^CGAA
SmlI 683 CTCAAG C^T[CT][GA]AG
Sse8387I 582 CCTGCAGG CCTGCA^GG
StuI 426 AGGCCT AGG^CCT
StyI 971 CCAAGG C^C[AT][AT]GG
TaqI 404 TCGA T^CGA
TfiI 95 GATTC G^A[AT]TC
TthHB8I 404 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 60 GGACC G^G[AT]CC
507 GGACC
BalI 162 TGGCCA TGG^CCA
512 TGGCCA
BbsI 270 GAAGAC GAAGAC(2/6)
400 GTCTTC
BfaI 148 CTAG C^TAG
1009 CTAG
BpuAI 270 GAAGAC GAAGAC(2/6)
400 GTCTTC
BsiYI 611 CCGAGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
969 CCCCAAGGGGG
BslI 611 CCGAGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
969 CCCCAAGGGGG
BspMI 409 ACCTGC ACCTGC(4/8)
581 ACCTGC
BsrDI 570 CATTGC GCAATG(2/0)
924 CATTGC
BsrI 803 CCAGT ACTGG(1/-1)
818 CCAGT
BstYI 679 GGATCT [GA]^GATC[CT]
780 AGATCC
EaeI 162 TGGCCA [CT]^GGCC[GA]
512 TGGCCA
HapII 47 CCGG C^CGG
475 CCGG
HgaI 621 GACGC GACGC(5/10)
724 GCGTC
HpaII 47 CCGG C^CGG
475 CCGG
MaeI 148 CTAG C^TAG
1009 CTAG
MaeIII 17 GTAAC ^GT[ACGT]AC
452 GTAAC
MflI 679 GGATCT [GA]^GATC[CT]
780 AGATCC
MluNI 162 TGGCCA TGG^CCA
512 TGGCCA
MscI 162 TGGCCA TGG^CCA
512 TGGCCA
MseI 359 TTAA T^TAA
658 TTAA
MspI 47 CCGG C^CGG
475 CCGG
PleI 349 GACTC GAGTC(4/5)
101 GAGTC
SfcI 296 CTACAG C^T[GA][CT]AG
583 CTGCAG
SspI 633 AATATT AAT^ATT
694 AATATT
Tru9I 359 TTAA T^TAA
658 TTAA
TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^
758 CAGTG
XbaI 147 TCTAGA T^CTAGA
1008 TCTAGA
XhoII 679 GGATCT [GA]^GATC[CT]
780 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsaJI BsgI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboI MboII MnlI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I