##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BcgI        
BciVI       BclI        BfaI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaBI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeI        MamI        MboI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcyI           42  GACGCC               G[GA]^CG[CT]C
AfaI          143  GTAC                 GT^AC
BbsI          559  GAAGAC               GAAGAC(2/6)
BbvI           30  GCTGC                GCAGC(8/12)
BcnI          447  CCGGG                CC^[GC]GG
BpuAI         559  GAAGAC               GAAGAC(2/6)
BsaAI         487  TACGTG               [CT]AC^GT[GA]
BsaHI          42  GACGCC               G[GA]^CG[CT]C
BsiEI          82  CGGTCG               CG[GA][CT]^CG
BsiYI         465  CCCTGACTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          465  CCCTGACTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         226  GTCTC                GTCTC(1/5)
BsmFI         449  GGGAC                GGGAC(10/14)
BsrI          129  ACTGG                ACTGG(1/-1)
BstEII        372  GGTAACC              G^GT[ACGT]ACC
BstPI         372  GGTAACC              G^GT[ACGT]ACC
EcoO65I       372  GGTAACC              G^GT[ACGT]ACC
EcoRI         133  GAATTC               G^AATTC
FauI          417  CCCGC                CCCGC(4/6)
Fnu4HI         30  GCTGC                GC^[ACGT]GC
HapII         447  CCGG                 C^CGG
HgaI           42  GACGC                GACGC(5/10)
Hin1I          42  GACGCC               G[GA]^CG[CT]C
HindIII       202  AAGCTT               A^AGCTT
HpaII         447  CCGG                 C^CGG
ItaI           30  GCTGC                GC^[ACGT]GC
MaeIII        373  GTAAC                ^GT[ACGT]AC
MfeI          363  CAATTG               C^AATTG
MspI          447  CCGG                 C^CGG
MunI          363  CAATTG               C^AATTG
MwoI          411  GCTCCTCCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          447  CCGGG                CC^[GC]GG
PshAI          73  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          143  GTAC                 GT^AC
SapI          676  GAAGAGC              GCTCTTC(1/4)
TfiI           18  GATTC                G^A[AT]TC
TseI           30  GCTGC                G^C[AT]GC
TspRI         323  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AciI          418  CCGC                 CCGC(-3/-1)
               33  GCGG                
AflII         138  CTTAAG               C^TTAAG
              196  CTTAAG              
BbvCI         306  CCTCAGC              CCTCAGC(-5/-2)
              478  GCTGAGG             
BfrI          138  CTTAAG               C^TTAAG
              196  CTTAAG              
EarI          595  GAAGAG               CTCTTC(1/4)
              676  GAAGAG              
Ksp632I       595  GAAGAG               CTCTTC(1/4)
              676  GAAGAG              
NlaIV         233  GGGGCC               GG[ACGT]^[ACGT]CC
              462  GGCCCC              
PleI          469  GACTC                GAGTC(4/5)
              224  GAGTC               
StuI          105  AGGCCT               AGG^CCT
              156  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        AluI        ApoI        BseRI       BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      FokI        HaeIII      HinfI       
HphI        MaeII       MboII       MnlI        MseI        
MvaI        Sau96I      ScrFI       SfaNI       SmlI        
TaqI        Tru9I       Tsp509I     TthHB8I