Restriction map for
pF1KB7263
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 231 GTCTAC GT^[AC][GT]AC
AcsI 911 GAATTC [GA]^AATT[CT]
AflIII 724 ACATGT A^C[GA][CT]GT
AlwI 279 GATCC GGATC(4/5)
ApoI 911 GAATTC [GA]^AATT[CT]
BalI 353 TGGCCA TGG^CCA
BanII 31 GAGCCC G[GA]GC[CT]^C
BbvI 705 GCAGC GCAGC(8/12)
BciVI 730 GTATCC GTATCC(6/5)
BlpI 301 GCTCAGC GC^T[ACGT]AGC
BmyI 31 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 301 GCTCAGC GC^T[ACGT]AGC
BseRI 936 GAGGAG GAGGAG(10/8)
BsgI 394 CTGCAC GTGCAG(16/14)
BsiYI 766 CCAGTTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 766 CCAGTTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 225 GTCCC GGGAC(10/14)
Bsp1286I 31 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 571 TGTACA T^GTACA
BspLU11I 724 ACATGT A^CATGT
BsrGI 571 TGTACA T^GTACA
BssSI 695 CTCGTG CACGAG(-5/-1)
BstXI 789 CCATCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 278 AGATCC [GA]^GATC[CT]
CelII 301 GCTCAGC GC^T[ACGT]AGC
DpnII 279 GATC ^GATC
DraIII 623 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 353 TGGCCA [CT]^GGCC[GA]
EcoRI 911 GAATTC G^AATTC
Fnu4HI 705 GCAGC GC^[ACGT]GC
HindIII 459 AAGCTT A^AGCTT
HphI 413 TCACC GGTGA(8/7)
ItaI 705 GCAGC GC^[ACGT]GC
MaeIII 133 GTTAC ^GT[ACGT]AC
MboI 279 GATC ^GATC
MflI 278 AGATCC [GA]^GATC[CT]
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MspA1I 304 CAGCTG C[AC]G^C[GT]G
MwoI 292 GCTTGCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 279 GATC ^GATC
NlaIV 30 GGAGCC GG[ACGT]^[ACGT]CC
NspI 724 ACATGT [GA]CATG^[CT]
PflMI 766 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GAGTC GAGTC(4/5)
PvuII 304 CAGCTG CAG^CTG
SapI 700 GAAGAGC GCTCTTC(1/4)
Sau3AI 279 GATC ^GATC
SfcI 879 CTATAG C^T[GA][CT]AG
SspBI 571 TGTACA T^GTACA
TseI 705 GCAGC G^C[AT]GC
Van91I 766 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 620 CCACACTAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 278 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 472 GTAC GT^AC
572 GTAC
AocI 45 CCTGAGG CC^T[ACGT]AGG
930 CCTAAGG
AvaII 584 GGACC G^G[AT]CC
786 GGTCC
BfaI 625 CTAG C^TAG
907 CTAG
BsrDI 615 CATTGC GCAATG(2/0)
518 GCAATG
Bsu36I 45 CCTGAGG CC^T[ACGT]AGG
930 CCTAAGG
Cfr13I 584 GGACC G^G[ACGT]CC
786 GGTCC
DsaI 356 CCATGG C^C[GA][CT]GG
513 CCATGG
EarI 94 CTCTTC CTCTTC(1/4)
700 GAAGAG
Eco81I 45 CCTGAGG CC^T[ACGT]AGG
930 CCTAAGG
EcoT14I 356 CCATGG C^C[AT][AT]GG
513 CCATGG
Ksp632I 94 CTCTTC CTCTTC(1/4)
700 GAAGAG
MaeI 625 CTAG C^TAG
907 CTAG
MseI 671 TTAA T^TAA
866 TTAA
NcoI 356 CCATGG C^CATGG
513 CCATGG
RsaI 472 GTAC GT^AC
572 GTAC
Sau96I 584 GGACC G^G[ACGT]CC
786 GGTCC
StyI 356 CCATGG C^C[AT][AT]GG
513 CCATGG
Tru9I 671 TTAA T^TAA
866 TTAA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BpmI BsaJI BsmAI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII FokI HaeIII HinfI MboII
MnlI MslI MvaI NlaIII ScrFI
SfaNI TfiI Tsp509I TspRI