Restriction map for
pF1KB7260
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrDI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AciI 787 GCGG CCGC(-3/-1)
AcsI 518 AAATTC [GA]^AATT[CT]
AgeI 362 ACCGGT A^CCGGT
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApoI 518 AAATTC [GA]^AATT[CT]
AvaII 771 GGTCC G^G[AT]CC
BalI 140 TGGCCA TGG^CCA
BamHI 57 GGATCC G^GATCC
BanII 896 GAGCCC G[GA]GC[CT]^C
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BbvI 922 GCAGC GCAGC(8/12)
BcgI 221 GCACTTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 398 TGATCA T^GATCA
BmyI 896 GAGCCC G[AGT]GC[ACT]^C
BpmI 553 CTCCAG CTGGAG(16/14)
BsaI 725 GGTCTC GGTCTC(1/5)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsgI 388 CTGCAC GTGCAG(16/14)
Bsp1286I 896 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 830 TGTACA T^GTACA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrGI 830 TGTACA T^GTACA
BstEII 840 GGTCACC G^GT[ACGT]ACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
BstYI 57 GGATCC [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
Cfr13I 771 GGTCC G^G[ACGT]CC
DsaI 815 CCGTGG C^C[GA][CT]GG
EaeI 140 TGGCCA [CT]^GGCC[GA]
EarI 798 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoNI 291 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
EcoT14I 633 CCTTGG C^C[AT][AT]GG
EcoT22I 490 ATGCAT ATGCA^T
FbaI 398 TGATCA T^GATCA
Fnu4HI 922 GCAGC GC^[ACGT]GC
HapII 363 CCGG C^CGG
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
HpaII 363 CCGG C^CGG
ItaI 922 GCAGC GC^[ACGT]GC
Ksp632I 798 CTCTTC CTCTTC(1/4)
MflI 57 GGATCC [GA]^GATC[CT]
MluNI 140 TGGCCA TGG^CCA
MscI 140 TGGCCA TGG^CCA
MspA1I 556 CAGCTG C[AC]G^C[GT]G
MspI 363 CCGG C^CGG
NsiI 490 ATGCAT ATGCA^T
PinAI 362 ACCGGT A^CCGGT
PvuII 556 CAGCTG CAG^CTG
Sau96I 771 GGTCC G^G[ACGT]CC
SexAI 104 ACCTGGT A^CC[AT]GGT
SspBI 830 TGTACA T^GTACA
StyI 633 CCTTGG C^C[AT][AT]GG
TseI 922 GCAGC G^C[AT]GC
TspRI 834 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 272 CCATCTCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 57 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 148 AGCT AG^CT
557 AGCT
Asp700I 659 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAACCCCTTC
BseRI 469 CTCCTC GAGGAG(10/8)
910 CTCCTC
BsmAI 726 GTCTC GTCTC(1/5)
908 GTCTC
BsmFI 757 GGGAC GGGAC(10/14)
232 GTCCC
BspHI 404 TCATGA T^CATGA
458 TCATGA
Cac8I 558 GCTGGC GC[ACGT]^[ACGT]GC
562 GCCTGC
MaeII 544 ACGT A^CGT
589 ACGT
RcaI 404 TCATGA T^CATGA
458 TCATGA
SfcI 67 CTACAG C^T[GA][CT]AG
867 CTACAG
Tsp509I 519 AATT ^AATT
660 AATT
XmnI 659 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AlwI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstXI DdeI
DpnII EcoRII FokI HaeIII HinfI
HphI MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI SfaNI
Tsp45I