Restriction map for
pF1KB7258
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
Hin1I HindIII HinfI HpaI ItaI
KasI KspI MfeI MluI MluNI
MroI MscI MspA1I MunI MvaI
MvnI MwoI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
TseI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 604 GGTACC G^GTACC
AcsI 25 GAATTC [GA]^AATT[CT]
ApoI 25 GAATTC [GA]^AATT[CT]
Asp718I 604 GGTACC G^GTACC
AvaII 502 GGACC G^G[AT]CC
BanI 604 GGTACC G^G[CT][GA]CC
BanII 51 GAGCCC G[GA]GC[CT]^C
BcnI 405 CCGGG CC^[GC]GG
BfaI 658 CTAG C^TAG
BglII 326 AGATCT A^GATCT
BmyI 51 GAGCCC G[AGT]GC[ACT]^C
BsaBI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 728 CCATGG C^C[ACGT][ACGT]GG
BseRI 121 CTCCTC GAGGAG(10/8)
BsgI 558 CTGCAC GTGCAG(16/14)
BsmAI 409 GAGAC GTCTC(1/5)
BsmFI 433 GGGAC GGGAC(10/14)
BsmI 879 GAATGC GAATGC(1/-1)
Bsp1286I 51 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 764 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrGI 764 TGTACA T^GTACA
BssKI 405 CCGGG ^CC[ACGT]GG
BstXI 608 CCATTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 326 AGATCT [GA]^GATC[CT]
DraIII 538 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 728 CCATGG C^C[GA][CT]GG
EarI 213 CTCTTC CTCTTC(1/4)
EcoRI 25 GAATTC G^AATTC
EcoT14I 728 CCATGG C^C[AT][AT]GG
HapII 405 CCGG C^CGG
HincII 241 GTTGAC GT[CT]^[GA]AC
HindII 241 GTTGAC GT[CT]^[GA]AC
HpaII 405 CCGG C^CGG
KpnI 604 GGTACC GGTAC^C
Ksp632I 213 CTCTTC CTCTTC(1/4)
MaeI 658 CTAG C^TAG
MamI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 326 AGATCT [GA]^GATC[CT]
MspI 405 CCGG C^CGG
NciI 405 CCGGG CC^[GC]GG
NcoI 728 CCATGG C^CATGG
RcaI 398 TCATGA T^CATGA
ScrFI 405 CCGGG CC^[ACGT]GG
SfaNI 650 GCATC GCATC(5/9)
SfcI 645 CTACAG C^T[GA][CT]AG
SspBI 764 TGTACA T^GTACA
StyI 728 CCATGG C^C[AT][AT]GG
Tsp509I 26 AATT ^AATT
XhoII 326 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 184 AGCT AG^CT
225 AGCT
BsiYI 272 CCTATGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
493 CCCTTTTGTGG
BslI 272 CCTATGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
493 CCCTTTTGTGG
BstF5I 333 CATCC GGATG(2/0)
654 CATCC
Cac8I 556 GCCTGC GC[ACGT]^[ACGT]GC
689 GCAGGC
CfoI 693 GCGC GCG^C
771 GCGC
Cfr13I 502 GGACC G^G[ACGT]CC
748 GGCCC
FokI 333 CATCC GGATG(9/13)
654 CATCC
HaeIII 348 GGCC GG^CC
748 GGCC
HhaI 693 GCGC GCG^C
771 GCGC
HinP1I 693 GCGC G^CGC
771 GCGC
HphI 163 TCACC GGTGA(8/7)
537 TCACC
MaeII 17 ACGT A^CGT
436 ACGT
MseI 158 TTAA T^TAA
838 TTAA
MslI 723 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
828 CACTCCCATG
Sau96I 502 GGACC G^G[ACGT]CC
748 GGCCC
Tru9I 158 TTAA T^TAA
838 TTAA
XcmI 266 CCATCTCCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
461 CCATTATCCAAGTGG
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AfaI BsrDI DdeI DpnII MaeIII
MboI MboII MnlI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Tsp45I
TspRI