Restriction map for
pF1KB7256
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
AspEI AspI AvaI AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KspI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 248 GGTACC G^GTACC
AfaI 249 GTAC GT^AC
AflIII 860 ACATGT A^C[GA][CT]GT
AlwI 1274 GGATC GGATC(4/5)
Aor51HI 664 AGCGCT AGC^GCT
Asp700I 574 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 248 GGTACC G^GTACC
AviII 704 TGCGCA TGC^GCA
BglI 334 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 314 AGATCT A^GATCT
BlpI 947 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 947 GCTTAGC GC^T[ACGT]AGC
BsaBI 67 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 564 GAGGAG GAGGAG(10/8)
BsgI 462 GTGCAG GTGCAG(16/14)
BsmI 524 GCATTC GAATGC(1/-1)
BspLU11I 860 ACATGT A^CATGT
BspMI 785 ACCTGC ACCTGC(4/8)
CelII 947 GCTTAGC GC^T[ACGT]AGC
DrdI 184 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 877 CCACGG C^C[GA][CT]GG
EarI 1246 CTCTTC CTCTTC(1/4)
Eco47III 664 AGCGCT AGC^GCT
EcoNI 557 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 53 GAATTC G^AATTC
EcoRV 349 GATATC GAT^ATC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
FauI 1229 CCCGC CCCGC(4/6)
FspI 704 TGCGCA TGC^GCA
HaeII 664 AGCGCT [GA]GCGC^[CT]
HincII 856 GTCAAC GT[CT]^[GA]AC
HindII 856 GTCAAC GT[CT]^[GA]AC
KpnI 248 GGTACC GGTAC^C
Ksp632I 1246 CTCTTC CTCTTC(1/4)
MaeIII 1084 GTAAC ^GT[ACGT]AC
MamI 67 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 476 CATATG CA^TATG
RsaI 249 GTAC GT^AC
SapI 1245 GCTCTTC GCTCTTC(1/4)
SexAI 869 ACCAGGT A^CC[AT]GGT
SspI 228 AATATT AAT^ATT
StuI 1048 AGGCCT AGG^CCT
StyI 161 CCAAGG C^C[AT][AT]GG
TaqI 1026 TCGA T^CGA
TthHB8I 1026 TCGA T^CGA
XmnI 574 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 175 GTCTAC GT^[AC][GT]AC
547 GTAGAC
AciI 1230 CCGC CCGC(-3/-1)
1316 GCGG
ApaI 341 GGGCCC GGGCC^C
1067 GGGCCC
BpmI 288 CTCCAG CTGGAG(16/14)
668 CTGGAG
BsaI 141 GAGACC GGTCTC(1/5)
453 GGTCTC
BsrI 155 ACTGG ACTGG(1/-1)
824 CCAGT
BstYI 314 AGATCT [GA]^GATC[CT]
1274 GGATCT
CfoI 665 GCGC GCG^C
705 GCGC
DpnII 315 GATC ^GATC
1275 GATC
DraII 341 GGGCCCC [GA]G^G[ACGT]CC[CT]
1066 AGGGCCC
DraIII 878 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1287 CACTCTGTG
EcoO109I 341 GGGCCCC [GA]G^G[ACGT]CC[CT]
1066 AGGGCCC
HhaI 665 GCGC GCG^C
705 GCGC
HinP1I 665 GCGC G^CGC
705 GCGC
HphI 699 GGTGA GGTGA(8/7)
917 TCACC
MaeII 545 ACGT A^CGT
1074 ACGT
MboI 315 GATC ^GATC
1275 GATC
MflI 314 AGATCT [GA]^GATC[CT]
1274 GGATCT
MseI 232 TTAA T^TAA
692 TTAA
MwoI 334 GCCATCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1069 GCCCAACGTGC
NdeII 315 GATC ^GATC
1275 GATC
NspI 293 GCATGT [GA]CATG^[CT]
860 ACATGT
PleI 732 GAGTC GAGTC(4/5)
1090 GAGTC
PstI 47 CTGCAG CTGCA^G
655 CTGCAG
Sau3AI 315 GATC ^GATC
1275 GATC
SfcI 47 CTGCAG C^T[GA][CT]AG
655 CTGCAG
Tru9I 232 TTAA T^TAA
692 TTAA
XcmI 170 CCACAGTCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
824 CCAGTGTGCTGGTGG
XhoII 314 AGATCT [GA]^GATC[CT]
1274 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI ApoI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboII MnlI
MslI MvaI NlaIII NlaIV Sau96I
ScrFI SfaNI TfiI TseI Tsp509I
TspRI