Restriction map for
pF1KB7255
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 205 GTAGAC GT^[AC][GT]AC
AciI 699 CCGC CCGC(-3/-1)
AcsI 98 GAATTT [GA]^AATT[CT]
AlwI 610 GGATC GGATC(4/5)
ApoI 98 GAATTT [GA]^AATT[CT]
Asp700I 586 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 758 GGACC G^G[AT]CC
BanII 896 GGGCTC G[GA]GC[CT]^C
BciVI 57 GGATAC GTATCC(6/5)
BclI 143 TGATCA T^GATCA
BfaI 94 CTAG C^TAG
BmyI 896 GGGCTC G[AGT]GC[ACT]^C
BpmI 413 CTGGAG CTGGAG(16/14)
BseRI 219 CTCCTC GAGGAG(10/8)
BsgI 720 CTGCAC GTGCAG(16/14)
BsiEI 326 CGGCCG CG[GA][CT]^CG
BsmI 462 GAATGC GAATGC(1/-1)
Bsp1286I 896 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 176 TGTACA T^GTACA
BspHI 245 TCATGA T^CATGA
BsrGI 176 TGTACA T^GTACA
BsrI 7 ACTGG ACTGG(1/-1)
BstYI 610 GGATCT [GA]^GATC[CT]
DraIII 627 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 326 CGGCCG C^GGCCG
EclXI 326 CGGCCG C^GGCCG
Eco52I 326 CGGCCG C^GGCCG
FbaI 143 TGATCA T^GATCA
HindIII 556 AAGCTT A^AGCTT
MaeI 94 CTAG C^TAG
MaeII 316 ACGT A^CGT
MflI 610 GGATCT [GA]^GATC[CT]
MwoI 346 GCTGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 350 CCATGG C^CATGG
NlaIV 757 GGGACC GG[ACGT]^[ACGT]CC
PleI 416 GAGTC GAGTC(4/5)
PstI 737 CTGCAG CTGCA^G
RcaI 245 TCATGA T^CATGA
SspBI 176 TGTACA T^GTACA
SspI 924 AATATT AAT^ATT
StuI 707 AGGCCT AGG^CCT
TfiI 843 GATTC G^A[AT]TC
XhoII 610 GGATCT [GA]^GATC[CT]
XmnI 586 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 255 CCTAAGG CC^T[ACGT]AGG
873 CCTCAGG
BalI 371 TGGCCA TGG^CCA
623 TGGCCA
BbvI 346 GCTGC GCAGC(8/12)
482 GCTGC
BspMI 400 ACCTGC ACCTGC(4/8)
718 ACCTGC
BssKI 412 CCTGG ^CC[ACGT]GG
447 CCTGG
BstF5I 672 CATCC GGATG(2/0)
454 GGATG
BstNI 412 CCTGG CC^[AT]GG
447 CCTGG
Bsu36I 255 CCTAAGG CC^T[ACGT]AGG
873 CCTCAGG
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
558 GCTTGC
DsaI 350 CCATGG C^C[GA][CT]GG
836 CCGTGG
EarI 603 CTCTTC CTCTTC(1/4)
903 GAAGAG
Eco81I 255 CCTAAGG CC^T[ACGT]AGG
873 CCTCAGG
EcoRII 412 CCTGG ^CC[AT]GG
447 CCTGG
EcoT14I 350 CCATGG C^C[AT][AT]GG
692 CCAAGG
FokI 672 CATCC GGATG(9/13)
454 GGATG
HinfI 416 GAGTC G^A[ACGT]TC
843 GATTC
Ksp632I 603 CTCTTC CTCTTC(1/4)
903 GAAGAG
MluNI 371 TGGCCA TGG^CCA
623 TGGCCA
MscI 371 TGGCCA TGG^CCA
623 TGGCCA
MvaI 412 CCTGG CC^[AT]GG
447 CCTGG
NspI 404 GCATGT [GA]CATG^[CT]
437 GCATGT
ScrFI 412 CCTGG CC^[ACGT]GG
447 CCTGG
StyI 350 CCATGG C^C[AT][AT]GG
692 CCAAGG
TseI 346 GCTGC G^C[AT]GC
482 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsmFI Cfr13I DdeI DpnII EaeI
Fnu4HI HaeIII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
NdeII NlaIII RsaI Sau3AI Sau96I
SfaNI SfcI Tru9I Tsp45I Tsp509I
TspRI