Restriction map for
pF1KB7252
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MboII MfeI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TaqI Tru9I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 1138 GAATTC [GA]^AATT[CT]
AcyI 3 GACGCC G[GA]^CG[CT]C
Alw44I 893 GTGCAC G^TGCAC
ApaLI 893 GTGCAC G^TGCAC
ApoI 1138 GAATTC [GA]^AATT[CT]
AspI 967 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 804 TGGCCA TGG^CCA
BbvCI 1160 CCTCAGC CCTCAGC(-5/-2)
BclI 113 TGATCA T^GATCA
BfaI 665 CTAG C^TAG
BglII 959 AGATCT A^GATCT
BsaHI 3 GACGCC G[GA]^CG[CT]C
BsaI 1251 GGTCTC GGTCTC(1/5)
BseRI 906 CTCCTC GAGGAG(10/8)
BsgI 61 GTGCAG GTGCAG(16/14)
BsmBI 972 CGTCTC CGTCTC(1/5)
BstYI 959 AGATCT [GA]^GATC[CT]
CfoI 798 GCGC GCG^C
DsaI 867 CCACGG C^C[GA][CT]GG
EaeI 804 TGGCCA [CT]^GGCC[GA]
EcoRI 1138 GAATTC G^AATTC
EcoRV 446 GATATC GAT^ATC
FbaI 113 TGATCA T^GATCA
HaeII 797 AGCGCC [GA]GCGC^[CT]
HhaI 798 GCGC GCG^C
Hin1I 3 GACGCC G[GA]^CG[CT]C
HinP1I 798 GCGC G^CGC
MaeI 665 CTAG C^TAG
MflI 959 AGATCT [GA]^GATC[CT]
MluNI 804 TGGCCA TGG^CCA
MscI 804 TGGCCA TGG^CCA
NspI 583 ACATGC [GA]CATG^[CT]
PflFI 967 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 117 CAGCTG CAG^CTG
SacI 1118 GAGCTC GAGCT^C
SbfI 687 CCTGCAGG CCTGCA^GG
SfaNI 1429 GATGC GCATC(5/9)
SmaI 611 CCCGGG CCC^GGG
Sse8387I 687 CCTGCAGG CCTGCA^GG
Tsp509I 1139 AATT ^AATT
Tth111I 967 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 959 AGATCT [GA]^GATC[CT]
XmaCI 611 CCCGGG C^CCGGG
XmaI 611 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 58 CGCG CG^CG
1170 CGCG
AspHI 893 GTGCAC G[AT]GC[AT]^C
1118 GAGCTC
AvaI 611 CCCGGG C^[CT]CG[GA]G
1381 CTCGGG
BlpI 97 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
BsiHKAI 893 GTGCAC G[AT]GC[AT]^C
1118 GAGCTC
BsmAI 973 GTCTC GTCTC(1/5)
1252 GTCTC
BsmFI 1174 GGGAC GGGAC(10/14)
621 GTCCC
BsoBI 611 CCCGGG C^[CT]CG[GA]G
1381 CTCGGG
BspMI 1214 ACCTGC ACCTGC(4/8)
1305 ACCTGC
BstUI 58 CGCG CG^CG
1170 CGCG
BstXI 389 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
795 CCAGCGCCCTGG
CelII 97 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
HgaI 3 GACGC GACGC(5/10)
1098 GCGTC
MaeII 783 ACGT A^CGT
1019 ACGT
MspA1I 117 CAGCTG C[AC]G^C[GT]G
288 CCGCTG
MvnI 58 CGCG CG^CG
1170 CGCG
PpuMI 52 AGGACCC [GA]G^G[AT]CC[CT]
1174 GGGACCT
PstI 308 CTGCAG CTGCA^G
688 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
ApaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TfiI TseI Tsp45I
TspRI XcmI