##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccII          92  CGCG                 CG^CG
AhdI          167  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         167  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI           8  GGATCC               G^GATCC
BbsI          278  GAAGAC               GAAGAC(2/6)
BpmI          196  CTCCAG               CTGGAG(16/14)
BpuAI         278  GAAGAC               GAAGAC(2/6)
BseRI          40  CTCCTC               GAGGAG(10/8)
BsgI          349  GTGCAG               GTGCAG(16/14)
BspMI         340  ACCTGC               ACCTGC(4/8)
BstF5I        249  GGATG                GGATG(2/0)
BstUI          92  CGCG                 CG^CG
BstXI         432  CCACAGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI           8  GGATCC               [GA]^GATC[CT]
DpnII           9  GATC                 ^GATC
Eam1105I      167  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI          249  GGATG                GGATG(9/13)
FseI          390  GGCCGGCC             GGCCGG^CC
HindIII       181  AAGCTT               A^AGCTT
HphI          186  TCACC                GGTGA(8/7)
MboI            9  GATC                 ^GATC
MboII         278  GAAGA                GAAGA(8/7)
MflI            8  GGATCC               [GA]^GATC[CT]
MslI          433  CACAGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           92  CGCG                 CG^CG
NdeII           9  GATC                 ^GATC
NlaIII        381  CATG                 CATG^
PleI          403  GACTC                GAGTC(4/5)
Sau3AI          9  GATC                 ^GATC
SfaNI          32  GCATC                GCATC(5/9)
TfiI          354  GAATC                G^A[AT]TC
XhoII           8  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI            9  GATCC                GGATC(4/5)
                8  GGATC               
AlwNI         310  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              407  CAGCTCCTG           
AvaI          267  CTCGGG               C^[CT]CG[GA]G
              285  CTCGGG              
BglI          193  GCCCTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              214  GCCGGCCTGGC         
BsoBI         267  CTCGGG               C^[CT]CG[GA]G
              285  CTCGGG              
DdeI          358  CTCAG                C^T[ACGT]AG
              405  CTCAG               
HinfI         354  GAATC                G^A[ACGT]TC
              403  GACTC               
PpuMI          86  GGGACCC              [GA]G^G[AT]CC[CT]
              298  AGGACCC             
PstI           49  CTGCAG               CTGCA^G
              142  CTGCAG              
SfcI           49  CTGCAG               C^T[GA][CT]AG
              142  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        ApaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiEI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrFI       BssKI       BstNI       Cac8I       Cfr10I      
Cfr13I      DraII       EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HpaII       ItaI        MnlI        
MspI        MvaI        MwoI        NaeI        NciI        
NgoMIV      NlaIV       Sau96I      ScrFI       TseI