##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwI          192  GGATC                GGATC(4/5)
Asp700I       264  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          38  GGTCC                G^G[AT]CC
BpmI           10  CTGGAG               CTGGAG(16/14)
BsaJI          62  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          364  GTGCAG               GTGCAG(16/14)
BsiYI          57  CCTATCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           57  CCTATCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssKI           9  CCTGG                ^CC[ACGT]GG
BstNI           9  CCTGG                CC^[AT]GG
BstYI         192  GGATCT               [GA]^GATC[CT]
Cac8I         351  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I         38  GGTCC                G^G[ACGT]CC
EcoNI          57  CCTATCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII          9  CCTGG                ^CC[AT]GG
EcoRV         220  GATATC               GAT^ATC
EcoT14I        62  CCAAGG               C^C[AT][AT]GG
HindIII       230  AAGCTT               A^AGCTT
HinfI          91  GATTC                G^A[ACGT]TC
MboII         264  GAAGA                GAAGA(8/7)
MflI          192  GGATCT               [GA]^GATC[CT]
MspA1I        349  CAGCTG               C[AC]G^C[GT]G
MvaI            9  CCTGG                CC^[AT]GG
MwoI          346  GCACAGCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         280  GGAACC               GG[ACGT]^[ACGT]CC
PstI          356  CTGCAG               CTGCA^G
PvuII         349  CAGCTG               CAG^CTG
Sau96I         38  GGTCC                G^G[ACGT]CC
ScaI          159  AGTACT               AGT^ACT
ScrFI           9  CCTGG                CC^[ACGT]GG
SfaNI         328  GATGC                GCATC(5/9)
SfcI          356  CTGCAG               C^T[GA][CT]AG
StyI           62  CCAAGG               C^C[AT][AT]GG
TfiI           91  GATTC                G^A[AT]TC
Tsp509I       144  AATT                 ^AATT
XhoII         192  GGATCT               [GA]^GATC[CT]
XmnI          264  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AfaI           54  GTAC                 GT^AC
              160  GTAC                
BsrI           44  ACTGG                ACTGG(1/-1)
              157  CCAGT               
DdeI          293  CTAAG                C^T[ACGT]AG
              303  CTCAG               
MnlI          166  CCTC                 CCTC(7/6)
              314  CCTC                
NlaIII        226  CATG                 CATG^
              321  CATG                
RsaI           54  GTAC                 GT^AC
              160  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AluI        BbvI        DpnII       Fnu4HI      ItaI        
MboI        NdeII       Sau3AI      TseI