Restriction map for
pF1KB7246
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 856 CGCG CG^CG
AflIII 788 ACACGT A^C[GA][CT]GT
Alw44I 1281 GTGCAC G^TGCAC
ApaI 20 GGGCCC GGGCC^C
ApaLI 1281 GTGCAC G^TGCAC
BalI 1151 TGGCCA TGG^CCA
BanI 137 GGTGCC G^G[CT][GA]CC
BglII 983 AGATCT A^GATCT
BsaBI 291 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 331 GAGACC GGTCTC(1/5)
BsmBI 418 GAGACG CGTCTC(1/5)
BspDI 298 ATCGAT AT^CGAT
BssSI 152 CTCGTG CACGAG(-5/-1)
BstAPI 429 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 856 CGCG CG^CG
BstXI 945 CCAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 983 AGATCT [GA]^GATC[CT]
ClaI 298 ATCGAT AT^CGAT
DsaI 806 CCATGG C^C[GA][CT]GG
EaeI 1151 TGGCCA [CT]^GGCC[GA]
EarI 754 GAAGAG CTCTTC(1/4)
EcoNI 651 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 1207 AAGCTT A^AGCTT
HphI 1553 TCACC GGTGA(8/7)
Ksp632I 754 GAAGAG CTCTTC(1/4)
MaeII 790 ACGT A^CGT
MamI 291 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 983 AGATCT [GA]^GATC[CT]
MluNI 1151 TGGCCA TGG^CCA
MscI 1151 TGGCCA TGG^CCA
MseI 122 TTAA T^TAA
MvnI 856 CGCG CG^CG
NcoI 806 CCATGG C^CATGG
SapI 754 GAAGAGC GCTCTTC(1/4)
SbfI 1056 CCTGCAGG CCTGCA^GG
Sse8387I 1056 CCTGCAGG CCTGCA^GG
Tru9I 122 TTAA T^TAA
Tsp509I 124 AATT ^AATT
XhoII 983 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 301 GATCC GGATC(4/5)
498 GGATC
BbsI 1521 GAAGAC GAAGAC(2/6)
1542 GAAGAC
BbvCI 892 GCTGAGG CCTCAGC(-5/-2)
898 GCTGAGG
BglI 140 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
461 GCCAGCAGGGC
BlpI 44 GCTCAGC GC^T[ACGT]AGC
1078 GCTGAGC
Bpu1102I 44 GCTCAGC GC^T[ACGT]AGC
1078 GCTGAGC
BpuAI 1521 GAAGAC GAAGAC(2/6)
1542 GAAGAC
BsgI 1401 CTGCAC GTGCAG(16/14)
1263 GTGCAG
BsmFI 572 GGGAC GGGAC(10/14)
956 GGGAC
CelII 44 GCTCAGC GC^T[ACGT]AGC
1078 GCTGAGC
DrdI 640 GACTTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1524 GACAGTTGAGTC
EcoT14I 806 CCATGG C^C[AT][AT]GG
1118 CCAAGG
HgaI 324 GACGC GACGC(5/10)
1108 GACGC
MspA1I 514 CAGCTG C[AC]G^C[GT]G
1500 CAGCTG
PvuII 514 CAGCTG CAG^CTG
1500 CAGCTG
StyI 806 CCATGG C^C[AT][AT]GG
1118 CCAAGG
TaqI 299 TCGA T^CGA
1534 TCGA
TthHB8I 299 TCGA T^CGA
1534 TCGA
XcmI 1154 CCAAGCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1427 CCAACATCATTCTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI Aor51HI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII Eco47III
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TfiI TseI Tsp45I TspRI