Restriction map for
pF1KB7242
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# NO CUT ENZYMES ( 193 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeI MamI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AciI 143 CCGC CCGC(-3/-1)
AcsI 524 GAATTT [GA]^AATT[CT]
AflIII 326 ACATGT A^C[GA][CT]GT
AlwI 166 GGATC GGATC(4/5)
AlwNI 313 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 524 GAATTT [GA]^AATT[CT]
AspHI 223 GTGCTC G[AT]GC[AT]^C
AvaII 258 GGACC G^G[AT]CC
BanII 505 GGGCTC G[GA]GC[CT]^C
BcgI 232 CGAAGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 377 TGATCA T^GATCA
BseRI 275 CTCCTC GAGGAG(10/8)
BsgI 536 GTGCAG GTGCAG(16/14)
BsiHKAI 223 GTGCTC G[AT]GC[AT]^C
BspLU11I 326 ACATGT A^CATGT
BstEII 407 GGTCACC G^GT[ACGT]ACC
BstPI 407 GGTCACC G^GT[ACGT]ACC
Cac8I 238 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 258 GGACC G^G[ACGT]CC
EcoO65I 407 GGTCACC G^GT[ACGT]ACC
FbaI 377 TGATCA T^GATCA
HinfI 370 GAGTC G^A[ACGT]TC
HphI 409 TCACC GGTGA(8/7)
MaeII 386 ACGT A^CGT
MfeI 122 CAATTG C^AATTG
MunI 122 CAATTG C^AATTG
NspI 326 ACATGT [GA]CATG^[CT]
PleI 370 GAGTC GAGTC(4/5)
Sau96I 258 GGACC G^G[ACGT]CC
TspRI 116 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 297 GTAC GT^AC
357 GTAC
BciVI 44 GGATAC GTATCC(6/5)
58 GGATAC
BmyI 223 GTGCTC G[AGT]GC[ACT]^C
505 GGGCTC
BsiYI 35 CCATCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
249 CCTGAAGATGG
BslI 35 CCATCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
249 CCTGAAGATGG
BsmAI 112 GAGAC GTCTC(1/5)
372 GTCTC
Bsp1286I 223 GTGCTC G[AGT]GC[ACT]^C
505 GGGCTC
BsrDI 210 CATTGC GCAATG(2/0)
101 GCAATG
DpnII 167 GATC ^GATC
378 GATC
EarI 174 CTCTTC CTCTTC(1/4)
233 GAAGAG
Ksp632I 174 CTCTTC CTCTTC(1/4)
233 GAAGAG
MaeIII 118 GTGAC ^GT[ACGT]AC
408 GTCAC
MboI 167 GATC ^GATC
378 GATC
MseI 71 TTAA T^TAA
152 TTAA
NdeII 167 GATC ^GATC
378 GATC
NlaIV 395 GGTTCC GG[ACGT]^[ACGT]CC
506 GGCTCC
RsaI 297 GTAC GT^AC
357 GTAC
SapI 233 GAAGAGC GCTCTTC(1/4)
173 GCTCTTC
Sau3AI 167 GATC ^GATC
378 GATC
SfaNI 214 GCATC GCATC(5/9)
515 GCATC
Tru9I 71 TTAA T^TAA
152 TTAA
Tsp45I 118 GTGAC ^GT[GC]AC
408 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BpmI BsaJI BssKI BstF5I BstNI
EcoRII FokI MboII MnlI MvaI
NlaIII ScrFI Tsp509I