##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MseI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII        142  TCCGGA               T^CCGGA
Alw44I         45  GTGCAC               G^TGCAC
AlwNI         309  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          45  GTGCAC               G^TGCAC
AvaII         251  GGTCC                G^G[AT]CC
BalI          330  TGGCCA               TGG^CCA
BbvCI         296  CCTCAGC              CCTCAGC(-5/-2)
BsaWI         142  TCCGGA               [AT]^CCGG[AT]
BseAI         142  TCCGGA               T^CCGGA
BsmAI         118  GTCTC                GTCTC(1/5)
BspEI         142  TCCGGA               T^CCGGA
BstXI         333  CCAGCACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        251  GGTCC                G^G[ACGT]CC
DraII         250  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          330  TGGCCA               [CT]^GGCC[GA]
EarI          120  CTCTTC               CTCTTC(1/4)
EcoO109I      250  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FauI          301  GCGGG                CCCGC(4/6)
HaeIII        331  GGCC                 GG^CC
HapII         143  CCGG                 C^CGG
HgaI          134  GCGTC                GACGC(5/10)
HpaII         143  CCGG                 C^CGG
Ksp632I       120  CTCTTC               CTCTTC(1/4)
MboII         121  TCTTC                GAAGA(8/7)
MluNI         330  TGGCCA               TGG^CCA
MroI          142  TCCGGA               T^CCGGA
MscI          330  TGGCCA               TGG^CCA
MslI           77  CACCGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          143  CCGG                 C^CGG
PpuMI         250  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         309  CAGCTG               CAG^CTG
SacI          273  GAGCTC               GAGCT^C
Sau96I        251  GGTCC                G^G[ACGT]CC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AspHI          45  GTGCAC               G[AT]GC[AT]^C
              273  GAGCTC              
BanII         273  GAGCTC               G[GA]GC[CT]^C
              303  GGGCTC              
BsiHKAI        45  GTGCAC               G[AT]GC[AT]^C
              273  GAGCTC              
BsiYI         161  CCAGCTCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              295  CCCTCAGCGGG         
BslI          161  CCAGCTCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              295  CCCTCAGCGGG         
BsmFI          98  GTCCC                GGGAC(10/14)
              252  GTCCC               
MspA1I        299  CAGCGG               C[AC]G^C[GT]G
              309  CAGCTG              
TspRI          25  CAGTG                CA[GC]TG[ACGT][ACGT]^
              327  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AluI        BbvI        BmyI        BpmI        
Bsp1286I    Cac8I       DdeI        Fnu4HI      ItaI        
MnlI        MwoI        NlaIV       PstI        SfcI        
TseI