Restriction map for
pF1KB7237
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BclI BfrI BglI BglII
BlnI BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI ItaI
KasI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 1423 ACATGT A^C[GA][CT]GT
Alw44I 475 GTGCAC G^TGCAC
AlwI 1317 GATCC GGATC(4/5)
ApaLI 475 GTGCAC G^TGCAC
Asp700I 1402 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1025 GGACC G^G[AT]CC
BbsI 1013 GAAGAC GAAGAC(2/6)
BcnI 405 CCGGG CC^[GC]GG
BlpI 229 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 229 GCTGAGC GC^T[ACGT]AGC
BpuAI 1013 GAAGAC GAAGAC(2/6)
BsaBI 739 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 187 GAGACC GGTCTC(1/5)
BsmFI 729 GGGAC GGGAC(10/14)
BsmI 713 GCATTC GAATGC(1/-1)
Bsp1407I 1426 TGTACA T^GTACA
BspLU11I 1423 ACATGT A^CATGT
BspMI 1370 GCAGGT ACCTGC(4/8)
BsrGI 1426 TGTACA T^GTACA
Bst1107I 204 GTATAC GTA^TAC
BstNI 769 CCTGG CC^[AT]GG
BstYI 1316 AGATCC [GA]^GATC[CT]
BstZ17I 204 GTATAC GTA^TAC
CelII 229 GCTGAGC GC^T[ACGT]AGC
EarI 1162 GAAGAG CTCTTC(1/4)
EcoRII 769 CCTGG ^CC[AT]GG
HaeIII 620 GGCC GG^CC
HapII 405 CCGG C^CGG
HincII 584 GTCAAC GT[CT]^[GA]AC
HindII 584 GTCAAC GT[CT]^[GA]AC
HpaII 405 CCGG C^CGG
HphI 64 TCACC GGTGA(8/7)
Ksp632I 1162 GAAGAG CTCTTC(1/4)
MaeIII 929 GTGAC ^GT[ACGT]AC
MamI 739 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1316 AGATCC [GA]^GATC[CT]
MspI 405 CCGG C^CGG
MvaI 769 CCTGG CC^[AT]GG
NciI 405 CCGGG CC^[GC]GG
PstI 1291 CTGCAG CTGCA^G
SacI 1281 GAGCTC GAGCT^C
SapI 1162 GAAGAGC GCTCTTC(1/4)
SfcI 1291 CTGCAG C^T[GA][CT]AG
SspBI 1426 TGTACA T^GTACA
SwaI 1131 ATTTAAAT ATTT^AAAT
Tsp45I 929 GTGAC ^GT[GC]AC
XcmI 1028 CCACTGTGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1316 AGATCC [GA]^GATC[CT]
XmnI 1402 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 204 GTATAC GT^[AC][GT]AC
394 GTAGAC
AcsI 130 GAATTC [GA]^AATT[CT]
797 GAATTC
ApoI 130 GAATTC [GA]^AATT[CT]
797 GAATTC
BciVI 307 GGATAC GTATCC(6/5)
1041 GGATAC
BfaI 88 CTAG C^TAG
911 CTAG
BpmI 630 CTCCAG CTGGAG(16/14)
399 CTGGAG
BsiYI 748 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
955 CCTCGGCTGGG
BslI 748 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
955 CCTCGGCTGGG
BsmAI 187 GAGAC GTCTC(1/5)
1323 GAGAC
BsrI 398 ACTGG ACTGG(1/-1)
316 CCAGT
BssKI 405 CCGGG ^CC[ACGT]GG
769 CCTGG
Cfr13I 619 GGGCC G^G[ACGT]CC
1025 GGACC
DpnII 104 GATC ^GATC
1317 GATC
EcoRI 130 GAATTC G^AATTC
797 GAATTC
MaeI 88 CTAG C^TAG
911 CTAG
MboI 104 GATC ^GATC
1317 GATC
NdeII 104 GATC ^GATC
1317 GATC
NlaIV 322 GGCTCC GG[ACGT]^[ACGT]CC
401 GGAGCC
NspI 1303 ACATGC [GA]CATG^[CT]
1423 ACATGT
PleI 697 GACTC GAGTC(4/5)
1325 GACTC
Sau3AI 104 GATC ^GATC
1317 GATC
Sau96I 619 GGGCC G^G[ACGT]CC
1025 GGACC
ScrFI 405 CCGGG CC^[ACGT]GG
769 CCTGG
SspI 1249 AATATT AAT^ATT
1378 AATATT
TfiI 154 GATTC G^A[AT]TC
1218 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI AspHI BanII BmyI
BsaJI BsiHKAI Bsp1286I BstF5I DdeI
DraI EcoT14I FokI HinfI MboII
MnlI MseI MslI NlaIII RsaI
SfaNI StyI Tru9I Tsp509I TspRI