Restriction map for
pF1KB7229
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 568 GTCGAC GT^[AC][GT]AC
AccII 244 CGCG CG^CG
AfaI 180 GTAC GT^AC
ApaI 66 GGGCCC GGGCC^C
AvaI 613 CCCGGG C^[CT]CG[GA]G
BalI 500 TGGCCA TGG^CCA
BanI 28 GGCACC G^G[CT][GA]CC
BanII 66 GGGCCC G[GA]GC[CT]^C
BbrPI 466 CACGTG CAC^GTG
BbsI 646 GTCTTC GAAGAC(2/6)
BfaI 709 CTAG C^TAG
BlpI 712 GCTGAGC GC^T[ACGT]AGC
BmyI 66 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 712 GCTGAGC GC^T[ACGT]AGC
BpuAI 646 GTCTTC GAAGAC(2/6)
BsaAI 466 CACGTG [CT]AC^GT[GA]
BsoBI 613 CCCGGG C^[CT]CG[GA]G
Bsp1286I 66 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 179 TGTACA T^GTACA
BspHI 863 TCATGA T^CATGA
BsrDI 284 GCAATG GCAATG(2/0)
BsrGI 179 TGTACA T^GTACA
BssSI 136 CACGAG CACGAG(-5/-1)
BstUI 244 CGCG CG^CG
BstXI 135 CCACGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 712 GCTGAGC GC^T[ACGT]AGC
CfoI 245 GCGC GCG^C
DpnII 942 GATC ^GATC
EaeI 500 TGGCCA [CT]^GGCC[GA]
FauI 206 CCCGC CCCGC(4/6)
HgaI 369 GCGTC GACGC(5/10)
HhaI 245 GCGC GCG^C
HinP1I 245 GCGC G^CGC
HincII 568 GTCGAC GT[CT]^[GA]AC
HindII 568 GTCGAC GT[CT]^[GA]AC
MaeI 709 CTAG C^TAG
MaeII 467 ACGT A^CGT
MboI 942 GATC ^GATC
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
MspA1I 768 CAGCTG C[AC]G^C[GT]G
MvnI 244 CGCG CG^CG
NdeII 942 GATC ^GATC
PmaCI 466 CACGTG CAC^GTG
PmlI 466 CACGTG CAC^GTG
PshAI 796 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 768 CAGCTG CAG^CTG
RcaI 863 TCATGA T^CATGA
RsaI 180 GTAC GT^AC
SalI 568 GTCGAC G^TCGAC
Sau3AI 942 GATC ^GATC
SexAI 313 ACCTGGT A^CC[AT]GGT
SmaI 613 CCCGGG CCC^GGG
SphI 152 GCATGC GCATG^C
SspBI 179 TGTACA T^GTACA
TfiI 252 GATTC G^A[AT]TC
XcmI 410 CCACCTTCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 613 CCCGGG C^CCGGG
XmaI 613 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BsaBI 252 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
678 GATATACATC
BsaI 90 GGTCTC GGTCTC(1/5)
574 GGTCTC
BseRI 346 GAGGAG GAGGAG(10/8)
379 GAGGAG
BsmAI 91 GTCTC GTCTC(1/5)
575 GTCTC
BsmFI 405 GTCCC GGGAC(10/14)
1041 GTCCC
BsmI 522 GAATGC GAATGC(1/-1)
595 GCATTC
BspMI 688 ACCTGC ACCTGC(4/8)
156 GCAGGT
BstEII 702 GGTCACC G^GT[ACGT]ACC
961 GGTGACC
BstF5I 303 CATCC GGATG(2/0)
642 GGATG
BstPI 702 GGTCACC G^GT[ACGT]ACC
961 GGTGACC
DraII 403 AGGTCCC [GA]G^G[ACGT]CC[CT]
719 AGGACCC
DraIII 493 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1099 CACCCTGTG
EarI 588 CTCTTC CTCTTC(1/4)
399 GAAGAG
EcoO109I 403 AGGTCCC [GA]G^G[ACGT]CC[CT]
719 AGGACCC
EcoO65I 702 GGTCACC G^GT[ACGT]ACC
961 GGTGACC
FokI 303 CATCC GGATG(9/13)
642 GGATG
HapII 32 CCGG C^CGG
614 CCGG
HpaII 32 CCGG C^CGG
614 CCGG
Ksp632I 588 CTCTTC CTCTTC(1/4)
399 GAAGAG
MamI 252 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
678 GATATACATC
MspI 32 CCGG C^CGG
614 CCGG
NcoI 95 CCATGG C^CATGG
555 CCATGG
NspI 152 GCATGC [GA]CATG^[CT]
903 GCATGT
PflMI 492 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
764 CCAACAGCTGG
PpuMI 403 AGGTCCC [GA]G^G[AT]CC[CT]
719 AGGACCC
Van91I 492 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
764 CCAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DsaI EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MslI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I