Restriction map for
pF1KB7220
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MseI
MspA1I MunI MvnI MwoI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 518 AAATTC [GA]^AATT[CT]
AlwI 538 GATCC GGATC(4/5)
ApoI 518 AAATTC [GA]^AATT[CT]
Asp700I 852 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 371 TGGCCA TGG^CCA
BclI 272 TGATCA T^GATCA
BcnI 413 CCCGG CC^[GC]GG
BfaI 800 CTAG C^TAG
BlpI 946 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 946 GCTAAGC GC^T[ACGT]AGC
BsaI 475 GAGACC GGTCTC(1/5)
BsmBI 923 CGTCTC CGTCTC(1/5)
Bsp1407I 827 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrGI 827 TGTACA T^GTACA
CelII 946 GCTAAGC GC^T[ACGT]AGC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 272 TGATCA T^GATCA
HapII 414 CCGG C^CGG
HpaII 414 CCGG C^CGG
MaeI 800 CTAG C^TAG
MaeII 922 ACGT A^CGT
MboII 182 TCTTC GAAGA(8/7)
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspI 414 CCGG C^CGG
NciI 413 CCCGG CC^[GC]GG
NspI 65 ACATGC [GA]CATG^[CT]
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 606 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 782 CTGCAG CTGCA^G
RcaI 404 TCATGA T^CATGA
SexAI 104 ACCTGGT A^CC[AT]GGT
SmlI 472 CTTGAG C^T[CT][GA]AG
SspBI 827 TGTACA T^GTACA
XcmI 15 CCAAACAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 852 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
523 CCGC
AocI 870 CCTGAGG CC^T[ACGT]AGG
912 CCTCAGG
AvaII 440 GGTCC G^G[AT]CC
938 GGACC
BsgI 388 CTGCAC GTGCAG(16/14)
504 CTGCAC
BsmFI 937 GGGAC GGGAC(10/14)
232 GTCCC
BsrDI 312 CATTGC GCAATG(2/0)
942 CATTGC
BsrI 757 ACTGG ACTGG(1/-1)
897 ACTGG
BstEII 108 GGTTACC G^GT[ACGT]ACC
837 GGTCACC
BstPI 108 GGTTACC G^GT[ACGT]ACC
837 GGTCACC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCATTGTCCTGG
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
912 CCTCAGG
Cfr13I 440 GGTCC G^G[ACGT]CC
938 GGACC
DpnII 273 GATC ^GATC
538 GATC
DsaI 81 CCATGG C^C[GA][CT]GG
833 CCATGG
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
912 CCTCAGG
EcoO65I 108 GGTTACC G^GT[ACGT]ACC
837 GGTCACC
MboI 273 GATC ^GATC
538 GATC
NcoI 81 CCATGG C^CATGG
833 CCATGG
NdeII 273 GATC ^GATC
538 GATC
Sau3AI 273 GATC ^GATC
538 GATC
Sau96I 440 GGTCC G^G[ACGT]CC
938 GGACC
SfcI 782 CTGCAG C^T[GA][CT]AG
864 CTACAG
TfiI 47 GATTC G^A[AT]TC
638 GAATC
Tsp509I 334 AATT ^AATT
519 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BseRI
BsiYI BslI BsmAI BssKI BstF5I
BstNI Cac8I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MnlI MslI MvaI
NlaIII NlaIV PleI RsaI ScrFI
SfaNI StyI TseI Tsp45I TspRI