Restriction map for
pF1KB7218
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbrPI BbsI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MluNI MscI MseI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 697 GTCTAC GT^[AC][GT]AC
AccIII 249 TCCGGA T^CCGGA
AcyI 686 GGCGCC G[GA]^CG[CT]C
ApaI 615 GGGCCC GGGCC^C
BamHI 728 GGATCC G^GATCC
BanI 686 GGCGCC G^G[CT][GA]CC
BbeI 686 GGCGCC GGCGC^C
BcgI 589 CGAGGTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 67 GCCATCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 664 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTGAGC GC^T[ACGT]AGC
BsaHI 686 GGCGCC G[GA]^CG[CT]C
BsaWI 249 TCCGGA [AT]^CCGG[AT]
BseAI 249 TCCGGA T^CCGGA
BseRI 13 CTCCTC GAGGAG(10/8)
BsgI 379 GTGCAG GTGCAG(16/14)
BsmFI 9 GGGAC GGGAC(10/14)
BspEI 249 TCCGGA T^CCGGA
BsrDI 629 GCAATG GCAATG(2/0)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BstF5I 504 CATCC GGATG(2/0)
BstYI 728 GGATCC [GA]^GATC[CT]
CelII 664 GCTGAGC GC^T[ACGT]AGC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
EcoNI 573 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 504 CATCC GGATG(9/13)
FseI 111 GGCCGGCC GGCCGG^CC
Hin1I 686 GGCGCC G[GA]^CG[CT]C
HphI 604 GGTGA GGTGA(8/7)
KasI 686 GGCGCC G^GCGCC
MaeII 363 ACGT A^CGT
MflI 728 GGATCC [GA]^GATC[CT]
MroI 249 TCCGGA T^CCGGA
NaeI 112 GCCGGC GCC^GGC
NarI 686 GGCGCC GG^CGCC
NgoMIV 112 GCCGGC G^CCGGC
PpuMI 302 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 402 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 484 CTGCAG CTGCA^G
SapI 135 GCTCTTC GCTCTTC(1/4)
SfaNI 503 GCATC GCATC(5/9)
SfcI 484 CTGCAG C^T[GA][CT]AG
SfoI 686 GGCGCC GGC^GCC
XcmI 600 CCAGGGTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 728 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 20 GTGCTC G[AT]GC[AT]^C
223 GAGCAC
BanII 615 GGGCCC G[GA]GC[CT]^C
667 GAGCCC
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
393 CCTCAGC
BcnI 264 CCGGG CC^[GC]GG
683 CCCGG
BpmI 675 CTCCAG CTGGAG(16/14)
508 CTGGAG
BsiHKAI 20 GTGCTC G[AT]GC[AT]^C
223 GAGCAC
BsmAI 409 GTCTC GTCTC(1/5)
428 GTCTC
DpnII 342 GATC ^GATC
729 GATC
EarI 118 CTCTTC CTCTTC(1/4)
136 CTCTTC
FauI 691 CCCGC CCCGC(4/6)
568 GCGGG
Ksp632I 118 CTCTTC CTCTTC(1/4)
136 CTCTTC
MboI 342 GATC ^GATC
729 GATC
MslI 322 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
482 CACTGCAGTG
NciI 264 CCGGG CC^[GC]GG
683 CCCGG
NdeII 342 GATC ^GATC
729 GATC
NspI 419 GCATGC [GA]CATG^[CT]
557 GCATGC
PvuII 179 CAGCTG CAG^CTG
256 CAGCTG
Sau3AI 342 GATC ^GATC
729 GATC
SphI 419 GCATGC GCATG^C
557 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI StyI TseI Tsp45I
TspRI