Restriction map for
pF1KB7216
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BseRI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MboI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 209 GTAC GT^AC
AhdI 68 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 9 AGCGCT AGC^GCT
ApaI 202 GGGCCC GGGCC^C
AspEI 68 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 259 GAGCTC G[AT]GC[AT]^C
AvaI 178 CTCGGG C^[CT]CG[GA]G
AvrII 111 CCTAGG C^CTAGG
BbvI 32 GCTGC GCAGC(8/12)
BlnI 111 CCTAGG C^CTAGG
BsaI 158 GGTCTC GGTCTC(1/5)
BsaWI 358 TCCGGT [AT]^CCGG[AT]
BsiHKAI 259 GAGCTC G[AT]GC[AT]^C
BsmBI 75 CGTCTC CGTCTC(1/5)
BsoBI 178 CTCGGG C^[CT]CG[GA]G
BsrI 282 CCAGT ACTGG(1/-1)
BssSI 54 CACGAG CACGAG(-5/-1)
BstF5I 271 GGATG GGATG(2/0)
Cac8I 59 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 10 GCGC GCG^C
DraIII 290 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 68 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 9 AGCGCT AGC^GCT
EcoNI 120 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 111 CCTAGG C^C[AT][AT]GG
FauI 80 CCCGC CCCGC(4/6)
Fnu4HI 32 GCTGC GC^[ACGT]GC
FokI 271 GGATG GGATG(9/13)
HaeII 9 AGCGCT [GA]GCGC^[CT]
HapII 359 CCGG C^CGG
HgaI 74 GCGTC GACGC(5/10)
HhaI 10 GCGC GCG^C
HinP1I 10 GCGC G^CGC
HindIII 139 AAGCTT A^AGCTT
HpaII 359 CCGG C^CGG
ItaI 32 GCTGC GC^[ACGT]GC
MaeIII 400 GTGAC ^GT[ACGT]AC
MspI 359 CCGG C^CGG
NlaIII 231 CATG CATG^
PpuMI 90 GGGACCC [GA]G^G[AT]CC[CT]
PstI 33 CTGCAG CTGCA^G
PvuII 367 CAGCTG CAG^CTG
RsaI 209 GTAC GT^AC
SacI 259 GAGCTC GAGCT^C
SfcI 33 CTGCAG C^T[GA][CT]AG
StyI 111 CCTAGG C^C[AT][AT]GG
TseI 32 GCTGC G^C[AT]GC
Tsp45I 400 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 7 enzymes) #
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AciI 30 CCGC CCGC(-3/-1)
81 CCGC
BanI 115 GGCACC G^G[CT][GA]CC
165 GGCACC
BpmI 85 CTCCAG CTGGAG(16/14)
280 CTCCAG
BsmFI 90 GGGAC GGGAC(10/14)
190 GGGAC
DraII 90 GGGACCC [GA]G^G[ACGT]CC[CT]
202 GGGCCCT
EcoO109I 90 GGGACCC [GA]G^G[ACGT]CC[CT]
202 GGGCCCT
MspA1I 30 CCGCTG C[AC]G^C[GT]G
367 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
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AluI AvaII BanII BfaI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI Cfr13I DdeI EcoRII
HaeIII HinfI HphI MaeI MnlI
MvaI MwoI NlaIV PleI Sau96I
ScrFI TspRI