Restriction map for
pF1KB7213
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstF5I BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraII DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI MvnI NarI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 209 GGCGTC G[GA]^CG[CT]C
AfaI 114 GTAC GT^AC
Alw44I 402 GTGCAC G^TGCAC
AlwNI 304 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 409 GGGCCC GGGCC^C
ApaLI 402 GTGCAC G^TGCAC
AspHI 402 GTGCAC G[AT]GC[AT]^C
BanI 361 GGCACC G^G[CT][GA]CC
BbrPI 13 CACGTG CAC^GTG
BpmI 214 CTGGAG CTGGAG(16/14)
BsaAI 13 CACGTG [CT]AC^GT[GA]
BsaHI 209 GGCGTC G[GA]^CG[CT]C
BsaJI 357 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 402 GTGCAC G[AT]GC[AT]^C
BstAPI 275 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 48 GGTCACC G^GT[ACGT]ACC
BstPI 48 GGTCACC G^GT[ACGT]ACC
CfoI 284 GCGC GCG^C
DpnII 81 GATC ^GATC
DrdI 93 GACAGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 157 TGGCCG [CT]^GGCC[GA]
EcoO65I 48 GGTCACC G^GT[ACGT]ACC
FauI 417 CCCGC CCCGC(4/6)
HgaI 210 GCGTC GACGC(5/10)
HhaI 284 GCGC GCG^C
Hin1I 209 GGCGTC G[GA]^CG[CT]C
HinP1I 284 GCGC G^CGC
MboI 81 GATC ^GATC
MboII 87 GAAGA GAAGA(8/7)
MspA1I 304 CAGCGG C[AC]G^C[GT]G
NaeI 286 GCCGGC GCC^GGC
NdeII 81 GATC ^GATC
NgoMIV 286 GCCGGC G^CCGGC
PflMI 12 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 13 CACGTG CAC^GTG
PmlI 13 CACGTG CAC^GTG
PstI 109 CTGCAG CTGCA^G
RsaI 114 GTAC GT^AC
Sau3AI 81 GATC ^GATC
SexAI 59 ACCTGGT A^CC[AT]GGT
SfaNI 140 GCATC GCATC(5/9)
SfcI 109 CTGCAG C^T[GA][CT]AG
TaqI 83 TCGA T^CGA
TspRI 154 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 83 TCGA T^CGA
Van91I 12 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 221 AGCT AG^CT
398 AGCT
AvaII 165 GGACC G^G[AT]CC
293 GGACC
BanII 232 GAGCCC G[GA]GC[CT]^C
409 GGGCCC
BcnI 53 CCGGG CC^[GC]GG
407 CCGGG
BspMI 298 ACCTGC ACCTGC(4/8)
313 ACCTGC
BsrFI 159 GCCGGT [GA]^CCGG[CT]
286 GCCGGC
Cac8I 286 GCCGGC GC[ACGT]^[ACGT]GC
302 GCCAGC
Cfr10I 159 GCCGGT [GA]^CCGG[CT]
286 GCCGGC
HaeIII 158 GGCC GG^CC
410 GGCC
HphI 75 GGTGA GGTGA(8/7)
50 TCACC
MaeII 14 ACGT A^CGT
270 ACGT
NciI 53 CCGGG CC^[GC]GG
407 CCGGG
Tsp45I 49 GTCAC ^GT[GC]AC
323 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BbvI BmyI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cfr13I
EcoRII Fnu4HI HapII HpaII ItaI
MaeIII MnlI MspI MvaI MwoI
NlaIV Sau96I ScrFI TseI