Restriction map for
pF1KB7206
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BbeI BbrPI BciVI
BclI BfrI BglII BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tru9I Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 856 GACGTC GACGT^C
AccI 622 GTCTAC GT^[AC][GT]AC
AflIII 1694 ACGTGT A^C[GA][CT]GT
Aor51HI 1368 AGCGCT AGC^GCT
ApaI 381 GGGCCC GGGCC^C
Asp700I 1231 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 514 GTGCTC G[AT]GC[AT]^C
AvrII 777 CCTAGG C^CTAGG
BamHI 1322 GGATCC G^GATCC
BanI 42 GGTGCC G^G[CT][GA]CC
BbsI 1518 GAAGAC GAAGAC(2/6)
BbvCI 534 CCTCAGC CCTCAGC(-5/-2)
BcgI 162 CGACAACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 777 CCTAGG C^CTAGG
BpuAI 1518 GAAGAC GAAGAC(2/6)
BsiHKAI 514 GTGCTC G[AT]GC[AT]^C
Bsp1407I 635 TGTACA T^GTACA
BspHI 1496 TCATGA T^CATGA
BsrFI 326 ACCGGC [GA]^CCGG[CT]
BsrGI 635 TGTACA T^GTACA
Cfr10I 326 ACCGGC [GA]^CCGG[CT]
DrdI 76 GACTTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1368 AGCGCT AGC^GCT
EcoRV 1210 GATATC GAT^ATC
EcoT22I 266 ATGCAT ATGCA^T
HaeII 1368 AGCGCT [GA]GCGC^[CT]
HgaI 1643 GCGTC GACGC(5/10)
NdeI 269 CATATG CA^TATG
NsiI 266 ATGCAT ATGCA^T
RcaI 1496 TCATGA T^CATGA
SapI 1231 GAAGAGC GCTCTTC(1/4)
SgrAI 325 CACCGGCG C[GA]^CCGG[CT]G
SmlI 208 CTTGAG C^T[CT][GA]AG
SspBI 635 TGTACA T^GTACA
XbaI 1200 TCTAGA T^CTAGA
XmnI 1231 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 1003 GAATTC [GA]^AATT[CT]
1459 GAATTC
AcyI 856 GACGTC G[GA]^CG[CT]C
1642 GGCGTC
AlwNI 305 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1327 CAGGTGCTG
ApoI 1003 GAATTC [GA]^AATT[CT]
1459 GAATTC
BcnI 610 CCCGG CC^[GC]GG
998 CCGGG
BfaI 778 CTAG C^TAG
1201 CTAG
BlpI 172 GCTCAGC GC^T[ACGT]AGC
1716 GCTGAGC
Bpu1102I 172 GCTCAGC GC^T[ACGT]AGC
1716 GCTGAGC
BsaHI 856 GACGTC G[GA]^CG[CT]C
1642 GGCGTC
BseRI 494 GAGGAG GAGGAG(10/8)
1046 GAGGAG
BsmI 264 GAATGC GAATGC(1/-1)
1606 GCATTC
BsrDI 1618 GCAATG GCAATG(2/0)
1679 GCAATG
BssSI 823 CTCGTG CACGAG(-5/-1)
925 CTCGTG
BstYI 203 AGATCC [GA]^GATC[CT]
1322 GGATCC
CelII 172 GCTCAGC GC^T[ACGT]AGC
1716 GCTGAGC
CfoI 1369 GCGC GCG^C
1721 GCGC
DsaI 725 CCATGG C^C[GA][CT]GG
1115 CCATGG
EarI 660 GAAGAG CTCTTC(1/4)
1231 GAAGAG
EcoNI 33 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1569 CCTCATGGAGG
EcoRI 1003 GAATTC G^AATTC
1459 GAATTC
FauI 987 CCCGC CCCGC(4/6)
1615 CCCGC
HhaI 1369 GCGC GCG^C
1721 GCGC
Hin1I 856 GACGTC G[GA]^CG[CT]C
1642 GGCGTC
HinP1I 1369 GCGC G^CGC
1721 GCGC
Ksp632I 660 GAAGAG CTCTTC(1/4)
1231 GAAGAG
MaeI 778 CTAG C^TAG
1201 CTAG
MflI 203 AGATCC [GA]^GATC[CT]
1322 GGATCC
NciI 610 CCCGG CC^[GC]GG
998 CCGGG
NcoI 725 CCATGG C^CATGG
1115 CCATGG
SfiI 277 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
439 GGCCAAGGTGGCC
SphI 410 GCATGC GCATG^C
1194 GCATGC
TaqI 1058 TCGA T^CGA
1670 TCGA
TfiI 931 GATTC G^A[AT]TC
939 GATTC
TthHB8I 1058 TCGA T^CGA
1670 TCGA
XhoII 203 AGATCC [GA]^GATC[CT]
1322 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanII BbvI BglI
BmyI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI XcmI