Restriction map for
pF1KB7203
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI AlwI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglII BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tsp509I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 967 TCCGGA T^CCGGA
AgeI 547 ACCGGT A^CCGGT
Alw44I 895 GTGCAC G^TGCAC
AlwNI 1501 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1661 AGCGCT AGC^GCT
ApaLI 895 GTGCAC G^TGCAC
AvrII 585 CCTAGG C^CTAGG
BbsI 1437 GAAGAC GAAGAC(2/6)
BclI 1595 TGATCA T^GATCA
BglI 715 GCCGGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 585 CCTAGG C^CTAGG
BpuAI 1437 GAAGAC GAAGAC(2/6)
BsaAI 704 CACGTA [CT]AC^GT[GA]
BsaI 544 GAGACC GGTCTC(1/5)
BseAI 967 TCCGGA T^CCGGA
BsmBI 525 CGTCTC CGTCTC(1/5)
BspEI 967 TCCGGA T^CCGGA
BspMI 410 ACCTGC ACCTGC(4/8)
BsrBI 199 GAGCGG CCGCTC(-3/-3)
BsrDI 1614 CATTGC GCAATG(2/0)
BsrFI 547 ACCGGT [GA]^CCGG[CT]
BssHII 451 GCGCGC G^CGCGC
BssSI 921 CACGAG CACGAG(-5/-1)
BstAPI 1404 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 547 ACCGGT [GA]^CCGG[CT]
DpnII 1596 GATC ^GATC
DraIII 1324 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1652 CTCTTC CTCTTC(1/4)
Eco47III 1661 AGCGCT AGC^GCT
FbaI 1595 TGATCA T^GATCA
HincII 1714 GTCAAC GT[CT]^[GA]AC
HindII 1714 GTCAAC GT[CT]^[GA]AC
Ksp632I 1652 CTCTTC CTCTTC(1/4)
MboI 1596 GATC ^GATC
MroI 967 TCCGGA T^CCGGA
MseI 884 TTAA T^TAA
NdeII 1596 GATC ^GATC
PflMI 93 CCATATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 547 ACCGGT A^CCGGT
SapI 1651 GCTCTTC GCTCTTC(1/4)
Sau3AI 1596 GATC ^GATC
SbfI 411 CCTGCAGG CCTGCA^GG
ScaI 1859 AGTACT AGT^ACT
SexAI 632 ACCTGGT A^CC[AT]GGT
SfiI 714 GGCCGGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1219 CTTGAG C^T[CT][GA]AG
Sse8387I 411 CCTGCAGG CCTGCA^GG
TaqI 479 TCGA T^CGA
Tru9I 884 TTAA T^TAA
TthHB8I 479 TCGA T^CGA
Van91I 93 CCATATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 890 GTAC GT^AC
1860 GTAC
BanI 1347 GGCACC G^G[CT][GA]CC
1509 GGTGCC
BbvCI 282 CCTCAGC CCTCAGC(-5/-2)
442 GCTGAGG
BfaI 586 CTAG C^TAG
1720 CTAG
BsaWI 547 ACCGGT [AT]^CCGG[AT]
967 TCCGGA
BsgI 1426 GTGCAG GTGCAG(16/14)
1498 GTGCAG
BstXI 1475 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1544 CCAATGAAGTGG
EcoT14I 585 CCTAGG C^C[AT][AT]GG
1334 CCTTGG
HaeII 375 GGCGCT [GA]GCGC^[CT]
1661 AGCGCT
MaeI 586 CTAG C^TAG
1720 CTAG
MaeII 705 ACGT A^CGT
1712 ACGT
MboII 1437 GAAGA GAAGA(8/7)
1653 TCTTC
RsaI 890 GTAC GT^AC
1860 GTAC
SmaI 56 CCCGGG CCC^GGG
343 CCCGGG
SphI 17 GCATGC GCATG^C
1409 GCATGC
StuI 230 AGGCCT AGG^CCT
1823 AGGCCT
StyI 585 CCTAGG C^C[AT][AT]GG
1334 CCTTGG
TfiI 1155 GATTC G^A[AT]TC
1850 GAATC
XmaCI 56 CCCGGG C^CCGGG
343 CCCGGG
XmaI 56 CCCGGG C^CCGGG
343 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII SacI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI
XcmI