Restriction map for
pF1KB7202
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbsI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 297 CGCG CG^CG
AfaI 350 GTAC GT^AC
AlwNI 424 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 41 GGGCCC GGGCC^C
BbeI 500 GGCGCC GGCGC^C
BbrPI 361 CACGTG CAC^GTG
BbvCI 654 CCTCAGC CCTCAGC(-5/-2)
BcgI 513 GCAACCGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 361 CACGTG [CT]AC^GT[GA]
BsgI 493 CTGCAC GTGCAG(16/14)
BsmAI 112 GTCTC GTCTC(1/5)
BsrBI 204 GAGCGG CCGCTC(-3/-3)
BsrFI 668 GCCGGC [GA]^CCGG[CT]
BssSI 334 CACGAG CACGAG(-5/-1)
BstUI 297 CGCG CG^CG
BstYI 636 GGATCT [GA]^GATC[CT]
Cfr10I 668 GCCGGC [GA]^CCGG[CT]
DdeI 655 CTCAG C^T[ACGT]AG
DsaI 405 CCATGG C^C[GA][CT]GG
EcoNI 606 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 176 GACGC GACGC(5/10)
HphI 90 GGTGA GGTGA(8/7)
KasI 500 GGCGCC G^GCGCC
MaeII 362 ACGT A^CGT
MflI 636 GGATCT [GA]^GATC[CT]
MvnI 297 CGCG CG^CG
NaeI 668 GCCGGC GCC^GGC
NarI 500 GGCGCC GG^CGCC
NcoI 405 CCATGG C^CATGG
NgoMIV 668 GCCGGC G^CCGGC
NlaIII 406 CATG CATG^
PaeR7I 36 CTCGAG C^TCGAG
PflMI 122 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 361 CACGTG CAC^GTG
PmlI 361 CACGTG CAC^GTG
PstI 13 CTGCAG CTGCA^G
PvuII 16 CAGCTG CAG^CTG
RsaI 350 GTAC GT^AC
SacI 283 GAGCTC GAGCT^C
SapI 583 GAAGAGC GCTCTTC(1/4)
SfaNI 694 GATGC GCATC(5/9)
SfcI 13 CTGCAG C^T[GA][CT]AG
SfoI 500 GGCGCC GGC^GCC
SmaI 394 CCCGGG CCC^GGG
TspRI 353 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 122 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 45 CCAAGGGAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 36 CTCGAG C^TCGAG
XhoII 636 GGATCT [GA]^GATC[CT]
XmaCI 394 CCCGGG C^CCGGG
XmaI 394 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 176 GACGCC G[GA]^CG[CT]C
500 GGCGCC
AspHI 283 GAGCTC G[AT]GC[AT]^C
457 GTGCTC
BanI 138 GGCACC G^G[CT][GA]CC
500 GGCGCC
BciVI 269 GTATCC GTATCC(6/5)
417 GTATCC
BfaI 533 CTAG C^TAG
649 CTAG
BglI 561 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
663 GCCCCGCCGGC
BpmI 229 CTCCAG CTGGAG(16/14)
280 CTGGAG
BsaHI 176 GACGCC G[GA]^CG[CT]C
500 GGCGCC
BseRI 286 CTCCTC GAGGAG(10/8)
107 GAGGAG
BsiHKAI 283 GAGCTC G[AT]GC[AT]^C
457 GTGCTC
BsrI 465 ACTGG ACTGG(1/-1)
571 CCAGT
BstF5I 118 CATCC GGATG(2/0)
123 CATCC
EaeI 72 CGGCCG [CT]^GGCC[GA]
243 CGGCCG
EagI 72 CGGCCG C^GGCCG
243 CGGCCG
EarI 198 CTCTTC CTCTTC(1/4)
583 GAAGAG
EclXI 72 CGGCCG C^GGCCG
243 CGGCCG
Eco52I 72 CGGCCG C^GGCCG
243 CGGCCG
EcoT14I 45 CCAAGG C^C[AT][AT]GG
405 CCATGG
FokI 118 CATCC GGATG(9/13)
123 CATCC
HaeII 445 GGCGCT [GA]GCGC^[CT]
500 GGCGCC
Hin1I 176 GACGCC G[GA]^CG[CT]C
500 GGCGCC
Ksp632I 198 CTCTTC CTCTTC(1/4)
583 GAAGAG
MaeI 533 CTAG C^TAG
649 CTAG
MboII 583 GAAGA GAAGA(8/7)
199 TCTTC
SmlI 36 CTCGAG C^T[CT][GA]AG
291 CTTGAG
StyI 45 CCAAGG C^C[AT][AT]GG
405 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI TaqI TfiI
TseI TthHB8I