Restriction map for
pF1KB7194
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaI BsiWI BsmI
BspDI BspLU11I BsrDI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
RsrII SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1618 GACGTC GACGT^C
AccIII 1187 TCCGGA T^CCGGA
AcsI 1582 AAATTC [GA]^AATT[CT]
AflIII 717 ACACGT A^C[GA][CT]GT
AocI 705 CCTGAGG CC^T[ACGT]AGG
Aor51HI 38 AGCGCT AGC^GCT
ApoI 1582 AAATTC [GA]^AATT[CT]
AspHI 1951 GAGCTC G[AT]GC[AT]^C
AviII 1661 TGCGCA TGC^GCA
BbrPI 1329 CACGTG CAC^GTG
BfaI 259 CTAG C^TAG
BglII 1178 AGATCT A^GATCT
BsaWI 1187 TCCGGA [AT]^CCGG[AT]
BseAI 1187 TCCGGA T^CCGGA
BsgI 1063 GTGCAG GTGCAG(16/14)
BsiHKAI 1951 GAGCTC G[AT]GC[AT]^C
Bsp1407I 797 TGTACA T^GTACA
BspEI 1187 TCCGGA T^CCGGA
BspHI 1436 TCATGA T^CATGA
BspMI 657 GCAGGT ACCTGC(4/8)
BsrBI 1348 GAGCGG CCGCTC(-3/-3)
BsrGI 797 TGTACA T^GTACA
BstXI 1692 CCAGTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 705 CCTGAGG CC^T[ACGT]AGG
DrdI 262 GACCCGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 38 AGCGCT AGC^GCT
Eco81I 705 CCTGAGG CC^T[ACGT]AGG
FspI 1661 TGCGCA TGC^GCA
MaeI 259 CTAG C^TAG
MroI 1187 TCCGGA T^CCGGA
MseI 1541 TTAA T^TAA
NaeI 532 GCCGGC GCC^GGC
NcoI 626 CCATGG C^CATGG
NgoMIV 532 GCCGGC G^CCGGC
NotI 243 GCGGCCGC GC^GGCCGC
PaeR7I 340 CTCGAG C^TCGAG
PmaCI 1329 CACGTG CAC^GTG
PmlI 1329 CACGTG CAC^GTG
PshAI 963 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1760 CGATCG CGAT^CG
RcaI 1436 TCATGA T^CATGA
SacI 1951 GAGCTC GAGCT^C
SgrAI 248 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 340 CTCGAG C^T[CT][GA]AG
SspBI 797 TGTACA T^GTACA
TfiI 1035 GATTC G^A[AT]TC
Tru9I 1541 TTAA T^TAA
Tsp509I 1583 AATT ^AATT
XcmI 1235 CCAAGAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 340 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 1904 GGGCCC GGGCC^C
2010 GGGCCC
AvaI 340 CTCGAG C^[CT]CG[GA]G
1044 CTCGGG
BcgI 435 CGAGCAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1754 GCAACACGATCG
BsaBI 474 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1179 GATCTACATC
BsmBI 270 CGTCTC CGTCTC(1/5)
1725 CGTCTC
BsoBI 340 CTCGAG C^[CT]CG[GA]G
1044 CTCGGG
BsrI 220 CCAGT ACTGG(1/-1)
1692 CCAGT
BssHII 143 GCGCGC G^CGCGC
304 GCGCGC
EagI 200 CGGCCG C^GGCCG
244 CGGCCG
EclXI 200 CGGCCG C^GGCCG
244 CGGCCG
Eco52I 200 CGGCCG C^GGCCG
244 CGGCCG
KspI 5 CCGCGG CCGC^GG
241 CCGCGG
MamI 474 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1179 GATCTACATC
MslI 1133 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1827 CACCGACATG
PflMI 350 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1809 CCAGAGGCTGG
PpuMI 177 AGGTCCC [GA]G^G[AT]CC[CT]
1460 AGGACCT
PstI 697 CTGCAG CTGCA^G
1675 CTGCAG
SacII 5 CCGCGG CCGC^GG
241 CCGCGG
ScaI 1562 AGTACT AGT^ACT
1631 AGTACT
SphI 1424 GCATGC GCATG^C
1556 GCATGC
Van91I 350 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1809 CCAGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI Asp700I AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SalI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII
XmnI