##################################
# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaJI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
Bsu36I Cfr10I Cfr13I ClaI CpoI
DpnII DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 369 GTATAC GT^[AC][GT]AC
AciI 578 GCGG CCGC(-3/-1)
AcsI 60 AAATTT [GA]^AATT[CT]
ApoI 60 AAATTT [GA]^AATT[CT]
Asp700I 734 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 799 GGCGCC GGCGC^C
BlpI 21 GCTCAGC GC^T[ACGT]AGC
BmyI 666 GGGCAC G[AGT]GC[ACT]^C
BpmI 620 CTGGAG CTGGAG(16/14)
Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC
BsaI 646 GAGACC GGTCTC(1/5)
BsaWI 649 ACCGGA [AT]^CCGG[AT]
BsmFI 860 GGGAC GGGAC(10/14)
Bsp1286I 666 GGGCAC G[AGT]GC[ACT]^C
BssKI 358 CCTGG ^CC[ACGT]GG
BssSI 854 CTCGTG CACGAG(-5/-1)
Bst1107I 369 GTATAC GTA^TAC
BstNI 358 CCTGG CC^[AT]GG
BstZ17I 369 GTATAC GTA^TAC
Cac8I 141 GCATGC GC[ACGT]^[ACGT]GC
CelII 21 GCTCAGC GC^T[ACGT]AGC
CfoI 800 GCGC GCG^C
EcoNI 358 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 358 CCTGG ^CC[AT]GG
HaeII 799 GGCGCC [GA]GCGC^[CT]
HapII 650 CCGG C^CGG
HgaI 349 GACGC GACGC(5/10)
HhaI 800 GCGC GCG^C
HinP1I 800 GCGC G^CGC
HpaII 650 CCGG C^CGG
KasI 799 GGCGCC G^GCGCC
MspA1I 576 CAGCGG C[AC]G^C[GT]G
MspI 650 CCGG C^CGG
MvaI 358 CCTGG CC^[AT]GG
NarI 799 GGCGCC GG^CGCC
NlaIII 142 CATG CATG^
NspI 141 GCATGC [GA]CATG^[CT]
ScrFI 358 CCTGG CC^[ACGT]GG
SfaNI 66 GCATC GCATC(5/9)
SfcI 729 CTATAG C^T[GA][CT]AG
SfoI 799 GGCGCC GGC^GCC
SphI 141 GCATGC GCATG^C
XmnI 734 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcyI 349 GACGCC G[GA]^CG[CT]C
799 GGCGCC
BsaHI 349 GACGCC G[GA]^CG[CT]C
799 GGCGCC
BstF5I 197 CATCC GGATG(2/0)
507 GGATG
DraI 162 TTTAAA TTT^AAA
898 TTTAAA
FokI 197 CATCC GGATG(9/13)
507 GGATG
HaeIII 39 GGCC GG^CC
119 GGCC
Hin1I 349 GACGCC G[GA]^CG[CT]C
799 GGCGCC
MaeIII 191 GTGAC ^GT[ACGT]AC
616 GTCAC
PflMI 691 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
745 CCACCTTTTGG
TfiI 88 GATTC G^A[AT]TC
277 GAATC
Tsp45I 191 GTGAC ^GT[GC]AC
616 GTCAC
TspRI 332 CACTG CA[GC]TG[ACGT][ACGT]^
618 CACTG
Van91I 691 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
745 CCACCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI AluI BanI BbsI BbvI
BpuAI BseRI BsiYI BslI BsmAI
BsrI DdeI EarI Fnu4HI HinfI
ItaI Ksp632I MboII MnlI MseI
MwoI NlaIV PleI RsaI TaqI
Tru9I TseI Tsp509I TthHB8I