Restriction map for
pF1KB7191
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI Alw44I AlwI
AocI ApaI ApaLI AscI Asp718I
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DsaI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AciI 1031 GCGG CCGC(-3/-1)
AclI 349 AACGTT AA^CGTT
AflII 1062 CTTAAG C^TTAAG
AhdI 556 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 970 AGCGCT AGC^GCT
AseI 693 ATTAAT AT^TAAT
AsnI 693 ATTAAT AT^TAAT
AspEI 556 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1563 GAGCTC G[AT]GC[AT]^C
AspI 1265 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 656 TGGCCA TGG^CCA
BanII 1563 GAGCTC G[GA]GC[CT]^C
BbsI 523 GTCTTC GAAGAC(2/6)
BbvCI 1548 GCTGAGG CCTCAGC(-5/-2)
BcnI 552 CCGGG CC^[GC]GG
BfrI 1062 CTTAAG C^TTAAG
BlpI 1565 GCTCAGC GC^T[ACGT]AGC
BmyI 1563 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 1565 GCTCAGC GC^T[ACGT]AGC
BpuAI 523 GTCTTC GAAGAC(2/6)
BseRI 547 CTCCTC GAGGAG(10/8)
BsiHKAI 1563 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1563 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 150 TGTACA T^GTACA
BspHI 131 TCATGA T^CATGA
BsrGI 150 TGTACA T^GTACA
CelII 1565 GCTCAGC GC^T[ACGT]AGC
DraI 1138 TTTAAA TTT^AAA
DrdI 514 GACACGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 656 TGGCCA [CT]^GGCC[GA]
Eam1105I 556 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 970 AGCGCT AGC^GCT
EcoT14I 1572 CCAAGG C^C[AT][AT]GG
EcoT22I 730 ATGCAT ATGCA^T
HaeII 970 AGCGCT [GA]GCGC^[CT]
HapII 552 CCGG C^CGG
HincII 34 GTTGAC GT[CT]^[GA]AC
HindII 34 GTTGAC GT[CT]^[GA]AC
HpaII 552 CCGG C^CGG
MaeII 350 ACGT A^CGT
MfeI 1463 CAATTG C^AATTG
MluNI 656 TGGCCA TGG^CCA
MscI 656 TGGCCA TGG^CCA
MspA1I 1010 CAGCTG C[AC]G^C[GT]G
MspI 552 CCGG C^CGG
MunI 1463 CAATTG C^AATTG
NciI 552 CCGGG CC^[GC]GG
NsiI 730 ATGCAT ATGCA^T
PflFI 1265 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 554 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 693 ATTAAT AT^TAAT
Psp1406I 349 AACGTT AA^CGTT
PstI 977 CTGCAG CTGCA^G
PvuII 1010 CAGCTG CAG^CTG
RcaI 131 TCATGA T^CATGA
SacI 1563 GAGCTC GAGCT^C
SfcI 977 CTGCAG C^T[GA][CT]AG
SphI 1369 GCATGC GCATG^C
SspBI 150 TGTACA T^GTACA
StuI 1380 AGGCCT AGG^CCT
StyI 1572 CCAAGG C^C[AT][AT]GG
TaqI 910 TCGA T^CGA
Tth111I 1265 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 910 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 215 GAATTT [GA]^AATT[CT]
405 AAATTC
AlwNI 293 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
641 CAGTTACTG
ApoI 215 GAATTT [GA]^AATT[CT]
405 AAATTC
Asp700I 215 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAGAACTTC
BfaI 905 CTAG C^TAG
1183 CTAG
BglII 250 AGATCT A^GATCT
1556 AGATCT
BpmI 290 CTCCAG CTGGAG(16/14)
1560 CTGGAG
BsaI 418 GAGACC GGTCTC(1/5)
1386 GGTCTC
BsaJI 8 CCCAGG C^C[ACGT][ACGT]GG
1572 CCAAGG
BsgI 575 GTGCAG GTGCAG(16/14)
1007 GTGCAG
BsiYI 1089 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1171 CCTGCCATTGG
BslI 1089 CCTTGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1171 CCTGCCATTGG
BsmFI 554 GGGAC GGGAC(10/14)
718 GGGAC
BssSI 994 CTCGTG CACGAG(-5/-1)
1213 CTCGTG
BstXI 1301 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCAAAGGCCTGG
BstYI 250 AGATCT [GA]^GATC[CT]
1556 AGATCT
CfoI 971 GCGC GCG^C
1373 GCGC
DraII 554 GGGACCC [GA]G^G[ACGT]CC[CT]
1484 AGGCCCT
EcoO109I 554 GGGACCC [GA]G^G[ACGT]CC[CT]
1484 AGGCCCT
HhaI 971 GCGC GCG^C
1373 GCGC
HinP1I 971 GCGC G^CGC
1373 GCGC
MaeI 905 CTAG C^TAG
1183 CTAG
MflI 250 AGATCT [GA]^GATC[CT]
1556 AGATCT
MslI 568 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
858 CATTGTAGTG
NspI 728 ACATGC [GA]CATG^[CT]
1369 GCATGC
PleI 603 GACTC GAGTC(4/5)
1269 GAGTC
SmlI 478 CTTGAG C^T[CT][GA]AG
1062 CTTAAG
SspI 769 AATATT AAT^ATT
1501 AATATT
XhoII 250 AGATCT [GA]^GATC[CT]
1556 AGATCT
XmnI 215 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI