##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI KpnI Ksp632I MaeI MaeII
MamI MboII MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 335 GGCGCC G[GA]^CG[CT]C
AluI 152 AGCT AG^CT
AlwI 389 GGATC GGATC(4/5)
AlwNI 100 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 214 GGGCCC GGGCC^C
AvaII 209 GGTCC G^G[AT]CC
BanI 335 GGCGCC G^G[CT][GA]CC
BanII 214 GGGCCC G[GA]GC[CT]^C
BbeI 335 GGCGCC GGCGC^C
BglI 296 GCCGCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 214 GGGCCC G[AGT]GC[ACT]^C
BpmI 226 CTCCAG CTGGAG(16/14)
BsaHI 335 GGCGCC G[GA]^CG[CT]C
BsaI 21 GGTCTC GGTCTC(1/5)
BsmAI 22 GTCTC GTCTC(1/5)
Bsp1286I 214 GGGCCC G[AGT]GC[ACT]^C
BsrFI 181 GCCGGC [GA]^CCGG[CT]
Cfr10I 181 GCCGGC [GA]^CCGG[CT]
DpnII 390 GATC ^GATC
DraII 214 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 246 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 214 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 287 CCATGG C^C[AT][AT]GG
HgaI 39 GACGC GACGC(5/10)
Hin1I 335 GGCGCC G[GA]^CG[CT]C
KasI 335 GGCGCC G^GCGCC
KspI 272 CCGCGG CCGC^GG
MaeIII 37 GTGAC ^GT[ACGT]AC
MboI 390 GATC ^GATC
NaeI 181 GCCGGC GCC^GGC
NarI 335 GGCGCC GG^CGCC
NcoI 287 CCATGG C^CATGG
NdeII 390 GATC ^GATC
NgoMIV 181 GCCGGC G^CCGGC
NlaIII 288 CATG CATG^
PshAI 112 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 272 CCGCGG CCGC^GG
Sau3AI 390 GATC ^GATC
SbfI 249 CCTGCAGG CCTGCA^GG
SfoI 335 GGCGCC GGC^GCC
SmaI 219 CCCGGG CCC^GGG
Sse8387I 249 CCTGCAGG CCTGCA^GG
StyI 287 CCATGG C^C[AT][AT]GG
Tsp45I 37 GTGAC ^GT[GC]AC
XmaCI 219 CCCGGG C^CCGGG
XmaI 219 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 219 CCCGGG C^[CT]CG[GA]G
331 CTCGGG
BsgI 95 CTGCAC GTGCAG(16/14)
157 CTGCAC
BsiEI 179 CGGCCG CG[GA][CT]^CG
353 CGGCCG
BsoBI 219 CCCGGG C^[CT]CG[GA]G
331 CTCGGG
BstF5I 186 CATCC GGATG(2/0)
279 CATCC
BstNI 26 CCTGG CC^[AT]GG
228 CCAGG
DsaI 272 CCGCGG C^C[GA][CT]GG
287 CCATGG
EaeI 179 CGGCCG [CT]^GGCC[GA]
353 CGGCCG
EagI 179 CGGCCG C^GGCCG
353 CGGCCG
EclXI 179 CGGCCG C^GGCCG
353 CGGCCG
Eco52I 179 CGGCCG C^GGCCG
353 CGGCCG
EcoRII 26 CCTGG ^CC[AT]GG
228 CCAGG
FokI 186 CATCC GGATG(9/13)
279 CATCC
MvaI 26 CCTGG CC^[AT]GG
228 CCAGG
PstI 238 CTGCAG CTGCA^G
250 CTGCAG
SfaNI 185 GCATC GCATC(5/9)
278 GCATC
SfcI 238 CTGCAG C^T[GA][CT]AG
250 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI BbvI BcnI BsaJI
BsiYI BslI BssKI BstUI Cac8I
CfoI Cfr13I FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI