Restriction map for
pF1KB7180
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI HphI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MunI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 259 ACGTGT A^C[GA][CT]GT
AlwI 191 GATCC GGATC(4/5)
AocI 426 CCTGAGG CC^T[ACGT]AGG
Aor51HI 794 AGCGCT AGC^GCT
AviII 825 TGCGCA TGC^GCA
BbeI 1173 GGCGCC GGCGC^C
BbsI 576 GAAGAC GAAGAC(2/6)
BcgI 1221 CGACCCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 490 GCTGAGC GC^T[ACGT]AGC
BpmI 244 CTGGAG CTGGAG(16/14)
Bpu1102I 490 GCTGAGC GC^T[ACGT]AGC
BpuAI 576 GAAGAC GAAGAC(2/6)
BseRI 919 GAGGAG GAGGAG(10/8)
BsgI 1212 CTGCAC GTGCAG(16/14)
BsmI 1091 GCATTC GAATGC(1/-1)
BssHII 145 GCGCGC G^CGCGC
BssSI 1226 CTCGTG CACGAG(-5/-1)
BstAPI 965 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 190 AGATCC [GA]^GATC[CT]
Bsu36I 426 CCTGAGG CC^T[ACGT]AGG
CelII 490 GCTGAGC GC^T[ACGT]AGC
Eco47III 794 AGCGCT AGC^GCT
Eco81I 426 CCTGAGG CC^T[ACGT]AGG
FseI 840 GGCCGGCC GGCCGG^CC
FspI 825 TGCGCA TGC^GCA
KasI 1173 GGCGCC G^GCGCC
MaeII 259 ACGT A^CGT
MaeIII 160 GTCAC ^GT[ACGT]AC
MflI 190 AGATCC [GA]^GATC[CT]
MseI 83 TTAA T^TAA
MslI 1092 CATTCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1173 GGCGCC GG^CGCC
NotI 1022 GCGGCCGC GC^GGCCGC
PflMI 188 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 287 AGGACCC [GA]G^G[AT]CC[CT]
PstI 885 CTGCAG CTGCA^G
SfcI 885 CTGCAG C^T[GA][CT]AG
SfoI 1173 GGCGCC GGC^GCC
SgrAI 356 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 225 CCCGGG CCC^GGG
Tru9I 83 TTAA T^TAA
Tsp45I 160 GTCAC ^GT[GC]AC
Tsp509I 619 AATT ^AATT
Van91I 188 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 539 CCATGGGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 190 AGATCC [GA]^GATC[CT]
XmaCI 225 CCCGGG C^CCGGG
XmaI 225 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 748 GTAC GT^AC
1246 GTAC
AlwNI 189 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
315 CAGCTTCTG
AspHI 310 GAGCAC G[AT]GC[AT]^C
1229 GTGCTC
AvaI 214 CCCGAG C^[CT]CG[GA]G
225 CCCGGG
BsiHKAI 310 GAGCAC G[AT]GC[AT]^C
1229 GTGCTC
BsmBI 513 GAGACG CGTCTC(1/5)
585 GAGACG
BsoBI 214 CCCGAG C^[CT]CG[GA]G
225 CCCGGG
BstXI 1055 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1170 CCAGGCGCCTGG
EarI 894 CTCTTC CTCTTC(1/4)
39 GAAGAG
EcoT14I 203 CCATGG C^C[AT][AT]GG
539 CCATGG
HaeII 794 AGCGCT [GA]GCGC^[CT]
1173 GGCGCC
Ksp632I 894 CTCTTC CTCTTC(1/4)
39 GAAGAG
KspI 1017 CCGCGG CCGC^GG
1084 CCGCGG
NaeI 357 GCCGGC GCC^GGC
841 GCCGGC
NcoI 203 CCATGG C^CATGG
539 CCATGG
NgoMIV 357 GCCGGC G^CCGGC
841 GCCGGC
PvuII 250 CAGCTG CAG^CTG
812 CAGCTG
RsaI 748 GTAC GT^AC
1246 GTAC
SacII 1017 CCGCGG CCGC^GG
1084 CCGCGG
SfiI 996 GGCCGTGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1015 GGCCGCGGCGGCC
StyI 203 CCATGG C^C[AT][AT]GG
539 CCATGG
TfiI 461 GAATC G^A[AT]TC
952 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I