Restriction map for
pF1KB7178
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BciVI BclI BcnI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoT22I FauI FbaI
FseI FspI HapII HincII HindII
HindIII HpaI HpaII KpnI KspI
MamI MluI MroI MspA1I MspI
MvnI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 308 GTAGAC GT^[AC][GT]AC
AcsI 57 AAATTT [GA]^AATT[CT]
AflII 411 CTTAAG C^TTAAG
AlwNI 925 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 653 GGGCCC GGGCC^C
ApoI 57 AAATTT [GA]^AATT[CT]
Asp700I 265 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 740 CCCGAG C^[CT]CG[GA]G
AvaII 285 GGACC G^G[AT]CC
BanII 653 GGGCCC G[GA]GC[CT]^C
BbeI 823 GGCGCC GGCGC^C
BcgI 729 CGAAGTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 200 CTAG C^TAG
BfrI 411 CTTAAG C^TTAAG
BglI 646 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 914 AGATCT A^GATCT
BpmI 32 CTGGAG CTGGAG(16/14)
BsaI 625 GAGACC GGTCTC(1/5)
BseRI 523 GAGGAG GAGGAG(10/8)
BsmBI 526 GAGACG CGTCTC(1/5)
BsmI 612 GAATGC GAATGC(1/-1)
BsoBI 740 CCCGAG C^[CT]CG[GA]G
Bsp1407I 439 TGTACA T^GTACA
BsrGI 439 TGTACA T^GTACA
BstAPI 804 GCACATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 882 GGTCACC G^GT[ACGT]ACC
BstF5I 864 CATCC GGATG(2/0)
BstPI 882 GGTCACC G^GT[ACGT]ACC
DdeI 911 CTGAG C^T[ACGT]AG
DraI 754 TTTAAA TTT^AAA
DraII 653 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1053 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 939 CCATGG C^C[GA][CT]GG
EcoNI 765 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 653 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 882 GGTCACC G^GT[ACGT]ACC
EcoRV 844 GATATC GAT^ATC
FokI 864 CATCC GGATG(9/13)
HgaI 528 GACGC GACGC(5/10)
HinfI 1146 GACTC G^A[ACGT]TC
KasI 823 GGCGCC G^GCGCC
MaeI 200 CTAG C^TAG
NarI 823 GGCGCC GG^CGCC
NcoI 939 CCATGG C^CATGG
NspI 707 GCATGC [GA]CATG^[CT]
PleI 1146 GACTC GAGTC(4/5)
SapI 1064 GAAGAGC GCTCTTC(1/4)
ScaI 1244 AGTACT AGT^ACT
SexAI 695 ACCTGGT A^CC[AT]GGT
SfcI 306 CTGTAG C^T[GA][CT]AG
SfoI 823 GGCGCC GGC^GCC
SphI 707 GCATGC GCATG^C
SspBI 439 TGTACA T^GTACA
XmnI 265 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 528 GACGCC G[GA]^CG[CT]C
823 GGCGCC
AlwI 111 GATCC GGATC(4/5)
372 GATCC
AspHI 688 GAGCAC G[AT]GC[AT]^C
796 GTGCTC
BanI 7 GGCACC G^G[CT][GA]CC
823 GGCGCC
BbrPI 334 CACGTG CAC^GTG
793 CACGTG
BsaHI 528 GACGCC G[GA]^CG[CT]C
823 GGCGCC
BsiHKAI 688 GAGCAC G[AT]GC[AT]^C
796 GTGCTC
BsmAI 526 GAGAC GTCTC(1/5)
625 GAGAC
BstXI 467 CCAAGCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAATCTCATGG
BstYI 371 AGATCC [GA]^GATC[CT]
914 AGATCT
EarI 329 CTCTTC CTCTTC(1/4)
1064 GAAGAG
EcoT14I 939 CCATGG C^C[AT][AT]GG
1141 CCAAGG
HaeII 69 GGCGCT [GA]GCGC^[CT]
823 GGCGCC
Hin1I 528 GACGCC G[GA]^CG[CT]C
823 GGCGCC
HphI 978 GGTGA GGTGA(8/7)
884 TCACC
Ksp632I 329 CTCTTC CTCTTC(1/4)
1064 GAAGAG
MflI 371 AGATCC [GA]^GATC[CT]
914 AGATCT
MslI 801 CATGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
885 CACCTCCGTG
PmaCI 334 CACGTG CAC^GTG
793 CACGTG
PmlI 334 CACGTG CAC^GTG
793 CACGTG
SmlI 64 CTTGAG C^T[CT][GA]AG
411 CTTAAG
StuI 231 AGGCCT AGG^CCT
986 AGGCCT
StyI 939 CCATGG C^C[AT][AT]GG
1141 CCAAGG
TaqI 149 TCGA T^CGA
1162 TCGA
TspRI 12 CACTG CA[GC]TG[ACGT][ACGT]^
135 CACTG
TthHB8I 149 TCGA T^CGA
1162 TCGA
XhoII 371 AGATCC [GA]^GATC[CT]
914 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI BalI BbsI
BbvI BmyI BpuAI BsaAI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DpnII
EaeI EcoRII Fnu4HI HaeIII HhaI
HinP1I ItaI MaeII MaeIII MboI
MboII MfeI MluNI MnlI MscI
MseI MunI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I