Restriction map for
pF1KB7169
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BanII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaHI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MfeI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
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Alw44I 289 GTGCAC G^TGCAC
ApaLI 289 GTGCAC G^TGCAC
BbsI 1197 GAAGAC GAAGAC(2/6)
BbvCI 441 GCTGAGG CCTCAGC(-5/-2)
BfaI 805 CTAG C^TAG
BglI 674 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1254 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1254 GCTAAGC GC^T[ACGT]AGC
BpuAI 1197 GAAGAC GAAGAC(2/6)
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaBI 99 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 116 CGGCCG CG[GA][CT]^CG
BsmBI 252 CGTCTC CGTCTC(1/5)
BsrDI 762 CATTGC GCAATG(2/0)
BsrFI 551 GCCGGT [GA]^CCGG[CT]
BsrI 8 ACTGG ACTGG(1/-1)
CelII 1254 GCTAAGC GC^T[ACGT]AGC
CfoI 315 GCGC GCG^C
Cfr10I 551 GCCGGT [GA]^CCGG[CT]
DraIII 283 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 116 CGGCCG C^GGCCG
EclXI 116 CGGCCG C^GGCCG
Eco52I 116 CGGCCG C^GGCCG
EcoNI 704 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 352 GCGGG CCCGC(4/6)
HgaI 383 GCGTC GACGC(5/10)
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
MaeI 805 CTAG C^TAG
MamI 99 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PleI 68 GAGTC GAGTC(4/5)
PvuII 1190 CAGCTG CAG^CTG
SfaNI 1234 GATGC GCATC(5/9)
SfiI 673 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 938 CCCGGG CCC^GGG
TaqI 899 TCGA T^CGA
TfiI 738 GATTC G^A[AT]TC
TthHB8I 899 TCGA T^CGA
XmaCI 938 CCCGGG C^CCGGG
XmaI 938 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 285 CGCG CG^CG
325 CGCG
AccIII 95 TCCGGA T^CCGGA
524 TCCGGA
AlwNI 66 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
724 CAGCCCCTG
AspHI 46 GAGCAC G[AT]GC[AT]^C
289 GTGCAC
AvaI 938 CCCGGG C^[CT]CG[GA]G
1030 CCCGAG
BpmI 9 CTGGAG CTGGAG(16/14)
427 CTGGAG
BsaI 862 GAGACC GGTCTC(1/5)
1117 GAGACC
BseAI 95 TCCGGA T^CCGGA
524 TCCGGA
BsiHKAI 46 GAGCAC G[AT]GC[AT]^C
289 GTGCAC
BsoBI 938 CCCGGG C^[CT]CG[GA]G
1030 CCCGAG
BspEI 95 TCCGGA T^CCGGA
524 TCCGGA
BstAPI 282 GCACGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1179 GCAAGGGCTGC
BstUI 285 CGCG CG^CG
325 CGCG
BstXI 168 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
566 CCAATGTGGTGG
DsaI 596 CCACGG C^C[GA][CT]GG
1070 CCACGG
EaeI 116 CGGCCG [CT]^GGCC[GA]
1091 TGGCCG
EcoT14I 108 CCTTGG C^C[AT][AT]GG
788 CCAAGG
HinfI 68 GAGTC G^A[ACGT]TC
738 GATTC
MroI 95 TCCGGA T^CCGGA
524 TCCGGA
MvnI 285 CGCG CG^CG
325 CGCG
NlaIII 303 CATG CATG^
624 CATG
SapI 390 GAAGAGC GCTCTTC(1/4)
1150 GAAGAGC
StuI 203 AGGCCT AGG^CCT
712 AGGCCT
StyI 108 CCTTGG C^C[AT][AT]GG
788 CCAAGG
TspRI 872 CACTG CA[GC]TG[ACGT][ACGT]^
956 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BbvI BcnI BmyI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TseI XhoII