Restriction map for
pF1KB7161
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AlwI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BamHI BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MflI MluI MroI MseI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 31 AACGTT AA^CGTT
AcsI 892 GAATTC [GA]^AATT[CT]
AhdI 394 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 382 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 196 CCTGAGG CC^T[ACGT]AGG
ApaI 600 GGGCCC GGGCC^C
ApoI 892 GAATTC [GA]^AATT[CT]
AspEI 394 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 673 CCCGAG C^[CT]CG[GA]G
AvrII 808 CCTAGG C^CTAGG
BalI 1070 TGGCCA TGG^CCA
BbsI 67 GAAGAC GAAGAC(2/6)
BciVI 366 GGATAC GTATCC(6/5)
BlnI 808 CCTAGG C^CTAGG
BpmI 850 CTGGAG CTGGAG(16/14)
BpuAI 67 GAAGAC GAAGAC(2/6)
BsaBI 507 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 808 CCTAGG C^C[ACGT][ACGT]GG
BsgI 835 GTGCAG GTGCAG(16/14)
BsmAI 1105 GTCTC GTCTC(1/5)
BsmBI 1104 CGTCTC CGTCTC(1/5)
BsmFI 840 GGGAC GGGAC(10/14)
BsoBI 673 CCCGAG C^[CT]CG[GA]G
BstAPI 1051 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1061 CCACTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
DpnII 708 GATC ^GATC
Eam1105I 394 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
EcoRI 892 GAATTC G^AATTC
EcoT14I 808 CCTAGG C^C[AT][AT]GG
HincII 34 GTTGAC GT[CT]^[GA]AC
HindII 34 GTTGAC GT[CT]^[GA]AC
HphI 641 TCACC GGTGA(8/7)
MaeIII 484 GTAAC ^GT[ACGT]AC
MamI 507 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 708 GATC ^GATC
MfeI 1040 CAATTG C^AATTG
MluNI 1070 TGGCCA TGG^CCA
MscI 1070 TGGCCA TGG^CCA
MunI 1040 CAATTG C^AATTG
NdeII 708 GATC ^GATC
NspI 668 GCATGC [GA]CATG^[CT]
Psp1406I 31 AACGTT AA^CGTT
Sau3AI 708 GATC ^GATC
ScaI 939 AGTACT AGT^ACT
SmlI 544 CTCAAG C^T[CT][GA]AG
SphI 668 GCATGC GCATG^C
StuI 636 AGGCCT AGG^CCT
StyI 808 CCTAGG C^C[AT][AT]GG
TfiI 183 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BbvI 350 GCAGC GCAGC(8/12)
308 GCTGC
BsmI 759 GAATGC GAATGC(1/-1)
245 GCATTC
Cac8I 386 GCCTGC GC[ACGT]^[ACGT]GC
668 GCATGC
EaeI 434 TGGCCG [CT]^GGCC[GA]
1070 TGGCCA
Fnu4HI 308 GCTGC GC^[ACGT]GC
350 GCAGC
ItaI 308 GCTGC GC^[ACGT]GC
350 GCAGC
MaeII 32 ACGT A^CGT
1103 ACGT
MslI 610 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1062 CACTGTCATG
PpuMI 267 GGGTCCT [GA]G^G[AT]CC[CT]
279 GGGTCCT
TseI 308 GCTGC G^C[AT]GC
350 GCAGC
Tsp509I 893 AATT ^AATT
1041 AATT
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BanII BfaI BmyI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DraII EarI EcoO109I EcoRII FokI
HaeIII HinfI Ksp632I MaeI MboII
MnlI MvaI MwoI NlaIII NlaIV
PleI RsaI SacI Sau96I ScrFI
SfaNI TspRI