Restriction map for
pF1KB7159
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 663 CTTAAG C^TTAAG
Alw44I 516 GTGCAC G^TGCAC
AlwNI 357 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 516 GTGCAC G^TGCAC
BamHI 791 GGATCC G^GATCC
BanI 142 GGCACC G^G[CT][GA]CC
BbsI 1045 GAAGAC GAAGAC(2/6)
BfrI 663 CTTAAG C^TTAAG
BglII 839 AGATCT A^GATCT
BpmI 477 CTGGAG CTGGAG(16/14)
BpuAI 1045 GAAGAC GAAGAC(2/6)
BsaBI 811 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 608 ACCGGA [AT]^CCGG[AT]
BsgI 315 CTGCAC GTGCAG(16/14)
BsmI 895 GCATTC GAATGC(1/-1)
BsrDI 345 CATTGC GCAATG(2/0)
BsrI 362 ACTGG ACTGG(1/-1)
CfoI 418 GCGC GCG^C
DraI 1063 TTTAAA TTT^AAA
EarI 396 GAAGAG CTCTTC(1/4)
HapII 609 CCGG C^CGG
HhaI 418 GCGC GCG^C
HinP1I 418 GCGC G^CGC
HindIII 265 AAGCTT A^AGCTT
HpaII 609 CCGG C^CGG
Ksp632I 396 GAAGAG CTCTTC(1/4)
MaeIII 196 GTGAC ^GT[ACGT]AC
MamI 811 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 609 CCGG C^CGG
PleI 81 GACTC GAGTC(4/5)
SfiI 405 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 663 CTTAAG C^T[CT][GA]AG
TaqI 803 TCGA T^CGA
Tsp45I 196 GTGAC ^GT[GC]AC
TthHB8I 803 TCGA T^CGA
XbaI 813 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 417 CGCG CG^CG
621 CGCG
AcsI 690 GAATTC [GA]^AATT[CT]
1005 GAATTC
ApoI 690 GAATTC [GA]^AATT[CT]
1005 GAATTC
AspHI 217 GTGCTC G[AT]GC[AT]^C
516 GTGCAC
AvaII 201 GGACC G^G[AT]CC
440 GGACC
BclI 128 TGATCA T^GATCA
1013 TGATCA
BglI 28 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
406 GCCAAGAGGGC
BmyI 217 GTGCTC G[AGT]GC[ACT]^C
516 GTGCAC
BsiHKAI 217 GTGCTC G[AT]GC[AT]^C
516 GTGCAC
Bsp1286I 217 GTGCTC G[AGT]GC[ACT]^C
516 GTGCAC
BspMI 310 ACCTGC ACCTGC(4/8)
319 ACCTGC
BstUI 417 CGCG CG^CG
621 CGCG
BstYI 791 GGATCC [GA]^GATC[CT]
839 AGATCT
EcoNI 279 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
782 CCTCCATCAGG
EcoRI 690 GAATTC G^AATTC
1005 GAATTC
FbaI 128 TGATCA T^GATCA
1013 TGATCA
MflI 791 GGATCC [GA]^GATC[CT]
839 AGATCT
MvnI 417 CGCG CG^CG
621 CGCG
NlaIV 142 GGCACC GG[ACGT]^[ACGT]CC
791 GGATCC
TfiI 597 GATTC G^A[AT]TC
885 GATTC
Tsp509I 691 AATT ^AATT
1006 AATT
XhoII 791 GGATCC [GA]^GATC[CT]
839 AGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI TspRI